A novel regulation of the oviductal glycoprotein gene expression by luteinizing hormone in bovine tubal epithelial cells.

The hypothesis that bovine oviductal epithelial cells contain luteinizing hormone (LH)/human chorionic gonadotropin (hCG) receptors and LH may regulate the oviductal glycoprotein (OGP) gene expression was tested. The results showed that bovine oviductal epithelial cells contain an 80 kDa LH/hCG receptor protein which can bind [(125)I]hCG. Culturing these cells with highly purified hCG, used as a surrogate hormone for LH, resulted in a dose and time dependent increase in a 95 kDa protein and a 2.3 kb transcript of OGP. The maximal increase of 100% was obtained with 10 ng/ml hCG and after three days of culture. LH, but not other hormones in the glycoprotein hormone family, isolated subunits of hCG, estradiol-17beta or progesterone, mimicked hCG, suggesting that the effect is hormone specific and requires the conformation of native hormone. The increase in steady state OGP mRNA levels by hCG is not due to an increase in the transcription rate of the gene. It is rather due to a significant increase in the half-life of OGP transcripts from 23 h in the control to 28 h after treatment with hCG. In summary, we conclude that LH and hCG can increase the synthesis of OGP by decreasing the degradation of its transcripts in bovine oviductal epithelial cells. Since OGP may play important roles in fertilization and growth and development of early embryo, higher LH levels present during the periovulatory period may directly participate in the early pregnancy events through increasing the synthesis of OGP.