Literature DB >> 3425902

Assay of reducing sugars in the nanomole range with 2,2'-bicinchoninate.

S Waffenschmidt1, L Jaenicke.   

Abstract

The photometric assay of reducing sugars with 2,2'-bicinchoninate was improved and its conditions were optimized. Optical density is linear between 1 and 25 nmol sugar/sample, and the assay is not affected by borate, phosphate, or other buffer anions. The molar extinction coefficients produced by the standard procedure with the 12 most common monosaccharides occurring in carbohydrates and conjugated glycocompounds are listed.

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Year:  1987        PMID： 3425902     DOI： 10.1016/0003-2697(87)90278-8

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  36 in total

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