OBJECTIVE: The study analyzed the prognostic value of the transcription of several tumor growth-related genes in gastric carcinoma biopsy specimens. SUMMARY BACKGROUND DATA: The nodal status is one of the most significant prognostic factors in gastric carcinoma. There are, however, no satisfactory parameters for the preoperative assessment of nodal status. METHODS: A reverse transcriptase-polymerase chain reaction analysis was used to analyze the transcription of several tumor growth-related genes in endoscopic biopsy specimens from 78 gastric carcinomas. The factors examined were cyclin D1, cyclin E, urokinase-type plasminogen activator, 72-kd type IV collagenase, vascular endothelial growth factor, platelet-derived growth factor-A (PDGF-A), transforming growth factor-beta, and interleukin-10. The relation between the mRNA expression and the clinical pathologic parameters was analyzed statistically. RESULTS: The incidence of PDGF-A (p = 0.010) and transforming growth factor-beta (p = 0.009) mRNA expression increased as the pathologic stage advanced. Nodal metastasis correlated with cyclin D1 (p = 0.045), cyclin E (p = 0.037), urokinase-type plasminogen activator (p = 0.047), and PDGF-A (p = 0.003) mRNA. Interestingly, the expression of PDGF-A mRNA showed a positive correlation (p = 0.004) with the early presence of lymph node metastases. CONCLUSIONS: Tumor growth-related factor mRNA in biopsy specimens may be a new prognostic tool.
OBJECTIVE: The study analyzed the prognostic value of the transcription of several tumor growth-related genes in gastric carcinoma biopsy specimens. SUMMARY BACKGROUND DATA: The nodal status is one of the most significant prognostic factors in gastric carcinoma. There are, however, no satisfactory parameters for the preoperative assessment of nodal status. METHODS: A reverse transcriptase-polymerase chain reaction analysis was used to analyze the transcription of several tumor growth-related genes in endoscopic biopsy specimens from 78 gastric carcinomas. The factors examined were cyclin D1, cyclin E, urokinase-type plasminogen activator, 72-kd type IV collagenase, vascular endothelial growth factor, platelet-derived growth factor-A (PDGF-A), transforming growth factor-beta, and interleukin-10. The relation between the mRNA expression and the clinical pathologic parameters was analyzed statistically. RESULTS: The incidence of PDGF-A (p = 0.010) and transforming growth factor-beta (p = 0.009) mRNA expression increased as the pathologic stage advanced. Nodal metastasis correlated with cyclin D1 (p = 0.045), cyclin E (p = 0.037), urokinase-type plasminogen activator (p = 0.047), and PDGF-A (p = 0.003) mRNA. Interestingly, the expression of PDGF-A mRNA showed a positive correlation (p = 0.004) with the early presence of lymph node metastases. CONCLUSIONS:Tumor growth-related factor mRNA in biopsy specimens may be a new prognostic tool.
Authors: T Toge; S Hamamoto; E Itagaki; K Yajima; M Tanada; H Nakane; H Kohno; K Nakanishi; T Hattori Journal: Cancer Date: 1983-11-01 Impact factor: 6.860
Authors: Carl C Schimanski; Friederike Schlaegel; Mareike Jordan; Markus Moehler; George Sgourakis; Daniel G Drescher; Peter R Galle; Hauke Lang; Ines Gockel Journal: World J Surg Date: 2011-05 Impact factor: 3.352
Authors: Megan O'Rourke; Carlie L Cullen; Loic Auderset; Kimberley A Pitman; Daniela Achatz; Robert Gasperini; Kaylene M Young Journal: PLoS One Date: 2016-09-14 Impact factor: 3.240