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Literature DB >> 9241430

Optical detection of single molecules.

Abstract

Recent advances in ultrasensitive instrumentation have allowed for the detection, identification, and dynamic studies of single molecules in the condensed phase. This measurement capability provides a new set of tools for scientists to address important current problems and to explore new frontiers in many scientific disciplines, such as chemistry, molecular biology, molecular medicine, and nanostructured materials. This review focuses on the methodologies and biological applications of single-molecule detection based on laser-induced fluorescence.

Mesh：

Substances：
Proteins
DNA

Year: 1997 PMID： 9241430 DOI： 10.1146/annurev.biophys.26.1.567

Source DB: PubMed Journal: Annu Rev Biophys Biomol Struct ISSN： 1056-8700

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Cited

52 in total

1. Ligand-induced conformational changes observed in single RNA molecules.

Authors: T Ha; X Zhuang; H D Kim; J W Orr; J R Williamson; S Chu
Journal: Proc Natl Acad Sci U S A Date: 1999-08-03 Impact factor: 11.205

2. Imaging and tracking of single GFP molecules in solution.

Authors: U Kubitscheck; O Kückmann; T Kues; R Peters
Journal: Biophys J Date: 2000-04 Impact factor: 4.033

3. Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives.

Authors: K Oiwa; J F Eccleston; M Anson; M Kikumoto; C T Davis; G P Reid; M A Ferenczi; J E Corrie; A Yamada; H Nakayama; D R Trentham
Journal: Biophys J Date: 2000-06 Impact factor: 4.033

4. Single-pair fluorescence resonance energy transfer on freely diffusing molecules: observation of Förster distance dependence and subpopulations.

Authors: A A Deniz; M Dahan; J R Grunwell; T Ha; A E Faulhaber; D S Chemla; S Weiss; P G Schultz
Journal: Proc Natl Acad Sci U S A Date: 1999-03-30 Impact factor: 11.205

5. Single-molecule spectroscopy of the beta(2) adrenergic receptor: observation of conformational substates in a membrane protein.

Authors: G Peleg; P Ghanouni; B K Kobilka; R N Zare
Journal: Proc Natl Acad Sci U S A Date: 2001-07-03 Impact factor: 11.205

6. High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking.

Authors: T Kues; A Dickmanns; R Lührmann; R Peters; U Kubitscheck
Journal: Proc Natl Acad Sci U S A Date: 2001-10-02 Impact factor: 11.205

Optical detection of single molecules.

1. Ligand-induced conformational changes observed in single RNA molecules.

2. Imaging and tracking of single GFP molecules in solution.

3. Comparative single-molecule and ensemble myosin enzymology: sulfoindocyanine ATP and ADP derivatives.

4. Single-pair fluorescence resonance energy transfer on freely diffusing molecules: observation of Förster distance dependence and subpopulations.

5. Single-molecule spectroscopy of the beta(2) adrenergic receptor: observation of conformational substates in a membrane protein.

6. High intranuclear mobility and dynamic clustering of the splicing factor U1 snRNP observed by single particle tracking.

7. Visualization and tracking of single protein molecules in the cell nucleus.

8. Simultaneous two-photon excitation of distinct labels for dual-color fluorescence crosscorrelation analysis.

9. Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites.

10. Information bounds and optimal analysis of dynamic single molecule measurements.