Literature DB >> 9237168

Purification of the transcription/repair factor TFIIH and evaluation of its associated activities in vitro.

J C Marinoni1, M Rossignol, J M Egly.   

Abstract

We describe here the methodology developed in our laboratory to study the role of TFIIH, a multisubunit protein complex, in the various mechanisms of cell life: transcription, DNA repair, and cell cycle regulation. Protocols are given to purify TFIIH and to study its various enzymatic activities as well as its transcription and nucleotide excision repair activities.

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Year:  1997        PMID： 9237168     DOI： 10.1006/meth.1997.0476

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  5 in total

1.  A family of chromatin remodeling factors related to Williams syndrome transcription factor.

Authors:  D A Bochar; J Savard; W Wang; D W Lafleur; P Moore; J Côté; R Shiekhattar
Journal:  Proc Natl Acad Sci U S A       Date:  2000-02-01       Impact factor: 11.205

2.  Strong functional interactions of TFIIH with XPC and XPG in human DNA nucleotide excision repair, without a preassembled repairosome.

Authors:  S J Araújo; E A Nigg; R D Wood
Journal:  Mol Cell Biol       Date:  2001-04       Impact factor: 4.272

3.  Nucleotide excision repair of DNA with recombinant human proteins: definition of the minimal set of factors, active forms of TFIIH, and modulation by CAK.

Authors:  S J Araújo; F Tirode; F Coin; H Pospiech; J E Syväoja; M Stucki; U Hübscher; J M Egly; R D Wood
Journal:  Genes Dev       Date:  2000-02-01       Impact factor: 11.361

4.  DNA repair and transcriptional deficiencies caused by mutations in the Drosophila p52 subunit of TFIIH generate developmental defects and chromosome fragility.

Authors:  Mariana Fregoso; Jean-Philippe Lainé; Javier Aguilar-Fuentes; Vincent Mocquet; Enrique Reynaud; Frédéric Coin; Jean-Marc Egly; Mario Zurita
Journal:  Mol Cell Biol       Date:  2007-03-05       Impact factor: 4.272

5.  Both XPD alleles contribute to the phenotype of compound heterozygote xeroderma pigmentosum patients.

Authors:  Takahiro Ueda; Emmanuel Compe; Philippe Catez; Kenneth H Kraemer; Jean-Marc Egly
Journal:  J Exp Med       Date:  2009-11-23       Impact factor: 14.307
  5 in total

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