Literature DB >> 9235997

The heptameric prepore of a staphylococcal alpha-hemolysin mutant in lipid bilayers imaged by atomic force microscopy.

Y Fang1, S Cheley, H Bayley, J Yang.   

Abstract

We have used atomic force microscopy to study the oligomeric state of a genetically engineered mutant of staphylococcal alpha-hemolysin (alphaHL-H5) that can be arrested as a "prepore" assembly intermediate. AFM images of alphaHL-H5 on supported bilayers of a fluid-phase lipid, egg-yolk phosphatidylcholine (egg-PC), under conditions that lock alphaHL-H5 into the prepore state, clearly show a heptameric structure for many individual oligomers. The central dent of the prepore has a diameter of 3.2 +/- 0.2 nm. The distance between the centers of mass of neighboring subunits is 2.8 +/- 0.3 nm. The heptamer has an average diameter of 8.9 +/- 0.6 nm. These results support a recently proposed pathway for the assembly of alpha-hemolysin.

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Year:  1997        PMID： 9235997     DOI： 10.1021/bi970600j

Source DB:  PubMed          Journal:  Biochemistry        ISSN： 0006-2960            Impact factor:   3.162


  15 in total

1.  Subunit composition of a bicomponent toxin: staphylococcal leukocidin forms an octameric transmembrane pore.

Authors:  George Miles; Liviu Movileanu; Hagan Bayley
Journal:  Protein Sci       Date:  2002-04       Impact factor: 6.725

2.  Arresting and releasing Staphylococcal alpha-hemolysin at intermediate stages of pore formation by engineered disulfide bonds.

Authors:  Toshimitsu Kawate; Eric Gouaux
Journal:  Protein Sci       Date:  2003-05       Impact factor: 6.725

3.  Properties of Bacillus cereus hemolysin II: a heptameric transmembrane pore.

Authors:  George Miles; Hagan Bayley; Stephen Cheley
Journal:  Protein Sci       Date:  2002-07       Impact factor: 6.725

4.  The leukocidin pore: evidence for an octamer with four LukF subunits and four LukS subunits alternating around a central axis.

Authors:  Lakmal Jayasinghe; Hagan Bayley
Journal:  Protein Sci       Date:  2005-10       Impact factor: 6.725

5.  Beyond Saffman-Delbruck approximation: a new regime for 2D diffusion of α-hemolysin complexes in supported lipid bilayer.

Authors:  Frédéric Harb; Joe Sarkis; Natalie Ferte; Bernard Tinland
Journal:  Eur Phys J E Soft Matter       Date:  2012-11-21       Impact factor: 1.890

Review 6.  Effects of MACPF/CDC proteins on lipid membranes.

Authors:  Robert J C Gilbert; Miha Mikelj; Mauro Dalla Serra; Christopher J Froelich; Gregor Anderluh
Journal:  Cell Mol Life Sci       Date:  2012-09-15       Impact factor: 9.261

Review 7.  Obstructing toxin pathways by targeted pore blockage.

Authors:  Ekaterina M Nestorovich; Sergey M Bezrukov
Journal:  Chem Rev       Date:  2012-10-11       Impact factor: 60.622

8.  Nanoscale imaging reveals laterally expanding antimicrobial pores in lipid bilayers.

Authors:  Paulina D Rakowska; Haibo Jiang; Santanu Ray; Alice Pyne; Baptiste Lamarre; Matthew Carr; Peter J Judge; Jascindra Ravi; Ulla I M Gerling; Beate Koksch; Glenn J Martyna; Bart W Hoogenboom; Anthony Watts; Jason Crain; Chris R M Grovenor; Maxim G Ryadnov
Journal:  Proc Natl Acad Sci U S A       Date:  2013-05-13       Impact factor: 11.205

9.  Membrane damage by an α-helical pore-forming protein, Equinatoxin II, proceeds through a succession of ordered steps.

Authors:  Nejc Rojko; Katarina Č Kristan; Gabriella Viero; Eva Žerovnik; Peter Maček; Mauro Dalla Serra; Gregor Anderluh
Journal:  J Biol Chem       Date:  2013-06-26       Impact factor: 5.157

10.  alpha-Hemolysin, gamma-hemolysin, and leukocidin from Staphylococcus aureus: distant in sequence but similar in structure.

Authors:  E Gouaux; M Hobaugh; L Song
Journal:  Protein Sci       Date:  1997-12       Impact factor: 6.725
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