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Literature DB >> 9226954

A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues.

J P Dalton¹, S R Day, A C Drew, P J Brindley.

Abstract

A novel method for the isolation of schistosome eggs and miracidia from livers of mice infected with Schistosoma japonicum or S. mansoni is described. The method employed collagenase B to degrade the interstitial matrix of mouse liver tissue, after which the schistosome eggs were separated from the liver cells by 2 single-step density centrifugations through Percoll. Using this procedure sufficient quantities of miracidia were obtained to generate a cDNA library. Southern blot analysis demonstrated that miracidia isolated by this method were free from contaminating host DNA.

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Year: 1997 PMID： 9226954 DOI： 10.1017/s0031182097001091

Source DB: PubMed Journal: Parasitology ISSN： 0031-1820 Impact factor: 3.234

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Cited

59 in total

1. Screening the Schistosoma mansoni transcriptome for genes differentially expressed in the schistosomulum stage in search for vaccine candidates.

Authors: Leonardo P Farias; Cibele A Tararam; Patricia A Miyasato; Milton Y Nishiyama; Katia C Oliveira; Toshie Kawano; Sergio Verjovski-Almeida; Luciana Cezar de Cerqueira Leite
Journal: Parasitol Res Date: 2010-09-18 Impact factor: 2.289

2. The embryonic development of Schistosoma mansoni eggs: proposal for a new staging system.

Authors: Arnon D Jurberg; Tiana Gonçalves; Tatiane A Costa; Ana Carolina A de Mattos; Bernardo M Pascarelli; Pedro Paulo A de Manso; Marcelo Ribeiro-Alves; Marcelo Pelajo-Machado; José M Peralta; Paulo Marcos Z Coelho; Henrique L Lenzi
Journal: Dev Genes Evol Date: 2009-05-05 Impact factor: 0.900

3. Reversible paralysis of Schistosoma mansoni by forchlorfenuron, a phenylurea cytokinin that affects septins.

Authors: Ana E Zeraik; Vitold E Galkin; Gabriel Rinaldi; Richard C Garratt; Michael J Smout; Alex Loukas; Victoria H Mann; Ana P U Araujo; Ricardo DeMarco; Paul J Brindley
Journal: Int J Parasitol Date: 2014-04-21 Impact factor: 3.981

4. Whole-genome sequence of Schistosoma haematobium.

Authors: Neil D Young; Aaron R Jex; Bo Li; Shiping Liu; Linfeng Yang; Zijun Xiong; Yingrui Li; Cinzia Cantacessi; Ross S Hall; Xun Xu; Fangyuan Chen; Xuan Wu; Adhemar Zerlotini; Guilherme Oliveira; Andreas Hofmann; Guojie Zhang; Xiaodong Fang; Yi Kang; Bronwyn E Campbell; Alex Loukas; Shoba Ranganathan; David Rollinson; Gabriel Rinaldi; Paul J Brindley; Huanming Yang; Jun Wang; Jian Wang; Robin B Gasser
Journal: Nat Genet Date: 2012-01-15 Impact factor: 38.330

5. A controlled human Schistosoma mansoni infection model to advance novel drugs, vaccines and diagnostics.

Authors: Marie-Astrid Hoogerwerf; Jan Pieter R Koopman; Marijke C C Langenberg; Jacqueline J Janse; Janneke Kos-van Oosterhoud; Carola Feijt; Simon P Jochems; Claudia J de Dood; Roos van Schuijlenburg; Arifa Ozir-Fazalalikhan; Mikhael D Manurung; Erliyani Sartono; Martha T van der Beek; Béatrice M F Winkel; Petra H Verbeek-Menken; Koen A Stam; Fijs W B van Leeuwen; Pauline Meij; Angela van Diepen; Lisette van Lieshout; Govert J van Dam; Paul L A M Corstjens; Cornelis H Hokke; Maria Yazdanbakhsh; Leo G Visser; Meta Roestenberg
Journal: Nat Med Date: 2020-02-17 Impact factor: 53.440

A method for the isolation of schistosome eggs and miracidia free of contaminating host tissues.

1. Screening the Schistosoma mansoni transcriptome for genes differentially expressed in the schistosomulum stage in search for vaccine candidates.

2. The embryonic development of Schistosoma mansoni eggs: proposal for a new staging system.

3. Reversible paralysis of Schistosoma mansoni by forchlorfenuron, a phenylurea cytokinin that affects septins.

4. Whole-genome sequence of Schistosoma haematobium.

5. A controlled human Schistosoma mansoni infection model to advance novel drugs, vaccines and diagnostics.

6. Electroporation facilitates introduction of reporter transgenes and virions into schistosome eggs.

7. A cytochrome b561 with ferric reductase activity from the parasitic blood fluke, Schistosoma japonicum.

8. Comparative real-time PCR and enzyme analysis of selected gender-associated molecules in Schistosoma japonicum.

Review 9. Culture for genetic manipulation of developmental stages of Schistosoma mansoni.

10. Tracking the fate of iron in early development of human blood flukes.