Literature DB >> 9214640

Localization of Sir2p: the nucleolus as a compartment for silent information regulators.

M Gotta1, S Strahl-Bolsinger, H Renauld, T Laroche, B K Kennedy, M Grunstein, S M Gasser.   

Abstract

In wild-type budding yeast strains, the proteins encoded by SIR3, SIR4 and RAP1 co-localize with telomeric DNA in a limited number of foci in interphase nuclei. Immunostaining of Sir2p shows that in addition to a punctate staining that coincides with Rap1 foci, Sir2p localizes to a subdomain of the nucleolus. The presence of Sir2p at both the spacer of the rDNA repeat and at telomeres is confirmed by formaldehyde cross-linking and immunoprecipitation with anti-Sir2p antibodies. In strains lacking Sir4p, Sir3p becomes concentrated in the nucleolus, by a pathway requiring SIR2 and UTH4, a gene that regulates life span in yeast. The unexpected nucleolar localization of Sir2p and Sir3p correlates with observed effects of sir mutations on rDNA stability and yeast longevity, defining a new site of action for silent information regulatory factors.

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Year:  1997        PMID： 9214640      PMCID： PMC1169941          DOI： 10.1093/emboj/16.11.3243

Source DB:  PubMed          Journal:  EMBO J        ISSN： 0261-4189            Impact factor:   11.598


  51 in total

1.  Telomeric position effect in yeast.

Authors:  L L Sandell; V A Zakian
Journal:  Trends Cell Biol       Date:  1992-01       Impact factor: 20.808

2.  Tandem gene amplification mediates copper resistance in yeast.

Authors:  S Fogel; J W Welch
Journal:  Proc Natl Acad Sci U S A       Date:  1982-09       Impact factor: 11.205

3.  The SIR1 gene of Saccharomyces cerevisiae and its role as an extragenic suppressor of several mating-defective mutants.

Authors:  E M Stone; M J Swanson; A M Romeo; J B Hicks; R Sternglanz
Journal:  Mol Cell Biol       Date:  1991-04       Impact factor: 4.272

4.  Separation of transcriptional activation and silencing functions of the RAP1-encoded repressor/activator protein 1: isolation of viable mutants affecting both silencing and telomere length.

Authors:  L Sussel; D Shore
Journal:  Proc Natl Acad Sci U S A       Date:  1991-09-01       Impact factor: 11.205

5.  Silent domains are assembled continuously from the telomere and are defined by promoter distance and strength, and by SIR3 dosage.

Authors:  H Renauld; O M Aparicio; P D Zierath; B L Billington; S K Chhablani; D E Gottschling
Journal:  Genes Dev       Date:  1993-07       Impact factor: 11.361

6.  Mutation in the silencing gene SIR4 can delay aging in S. cerevisiae.

Authors:  B K Kennedy; N R Austriaco; J Zhang; L Guarente
Journal:  Cell       Date:  1995-02-10       Impact factor: 41.582

7.  Modifiers of position effect are shared between telomeric and silent mating-type loci in S. cerevisiae.

Authors:  O M Aparicio; B L Billington; D E Gottschling
Journal:  Cell       Date:  1991-09-20       Impact factor: 41.582

8.  Mapping Polycomb-repressed domains in the bithorax complex using in vivo formaldehyde cross-linked chromatin.

Authors:  V Orlando; R Paro
Journal:  Cell       Date:  1993-12-17       Impact factor: 41.582

9.  Histone H3 and H4 N-termini interact with SIR3 and SIR4 proteins: a molecular model for the formation of heterochromatin in yeast.

Authors:  A Hecht; T Laroche; S Strahl-Bolsinger; S M Gasser; M Grunstein
Journal:  Cell       Date:  1995-02-24       Impact factor: 41.582

10.  The clustering of telomeres and colocalization with Rap1, Sir3, and Sir4 proteins in wild-type Saccharomyces cerevisiae.

Authors:  M Gotta; T Laroche; A Formenton; L Maillet; H Scherthan; S M Gasser
Journal:  J Cell Biol       Date:  1996-09       Impact factor: 10.539
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  105 in total

1.  On the mechanism of silencing in Escherichia coli.

Authors:  J Rine
Journal:  Proc Natl Acad Sci U S A       Date:  1999-07-20       Impact factor: 11.205

2.  SAS4 and SAS5 are locus-specific regulators of silencing in Saccharomyces cerevisiae.

Authors:  E Y Xu; S Kim; D H Rivier
Journal:  Genetics       Date:  1999-09       Impact factor: 4.562

3.  Analysis of Sir2p domains required for rDNA and telomeric silencing in Saccharomyces cerevisiae.

Authors:  M M Cockell; S Perrod; S M Gasser
Journal:  Genetics       Date:  2000-03       Impact factor: 4.562

4.  Cohabitation of insulators and silencing elements in yeast subtelomeric regions.

Authors:  G Fourel; E Revardel; C E Koering; E Gilson
Journal:  EMBO J       Date:  1999-05-04       Impact factor: 11.598

5.  Limitations of silencing at native yeast telomeres.

Authors:  F E Pryde; E J Louis
Journal:  EMBO J       Date:  1999-05-04       Impact factor: 11.598

6.  Chromosomal position effects reveal different cis-acting requirements for rDNA transcription and sex chromosome pairing in Drosophila melanogaster.

Authors:  A Briscoe; J E Tomkiel
Journal:  Genetics       Date:  2000-07       Impact factor: 4.562

7.  Terminal retrotransposons activate a subtelomeric white transgene at the 2L telomere in Drosophila.

Authors:  M D Golubovsky; A Y Konev; M F Walter; H Biessmann; J M Mason
Journal:  Genetics       Date:  2001-07       Impact factor: 4.562

8.  Dicentric chromosome stretching during anaphase reveals roles of Sir2/Ku in chromatin compaction in budding yeast.

Authors:  D A Thrower; K Bloom
Journal:  Mol Biol Cell       Date:  2001-09       Impact factor: 4.138

9.  Role for the silencing protein Dot1 in meiotic checkpoint control.

Authors:  P A San-Segundo; G S Roeder
Journal:  Mol Biol Cell       Date:  2000-10       Impact factor: 4.138

10.  Locus specificity determinants in the multifunctional yeast silencing protein Sir2.

Authors:  G Cuperus; R Shafaatian; D Shore
Journal:  EMBO J       Date:  2000-06-01       Impact factor: 11.598
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