Literature DB >> 9185569

Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP).

N W Kim1, F Wu.   

Abstract

The telomeric repeat amplification protocol (TRAP) assay has been used to test telomerase activity in numerous cancer specimens. We describe primers, controls and quantification methods for the TRAP assay to accurately measure the level of telomerase activity in clinical samples. The assay is reliable and reproducible in routine analyses and can be used to estimate the processivity of telomerase activity.

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Year:  1997        PMID： 9185569      PMCID： PMC146790          DOI： 10.1093/nar/25.13.2595

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  5 in total

1.  Identification of a specific telomere terminal transferase activity in Tetrahymena extracts.

Authors:  C W Greider; E H Blackburn
Journal:  Cell       Date:  1985-12       Impact factor: 41.582

Review 2.  Telomerase, cell immortality, and cancer.

Authors:  C B Harley; N W Kim; K R Prowse; S L Weinrich; K S Hirsch; M D West; S Bacchetti; H W Hirte; C M Counter; C W Greider
Journal:  Cold Spring Harb Symp Quant Biol       Date:  1994

3.  Modifications of a telomeric repeat amplification protocol (TRAP) result in increased reliability, linearity and sensitivity.

Authors:  W E Wright; J W Shay; M A Piatyszek
Journal:  Nucleic Acids Res       Date:  1995-09-25       Impact factor: 16.971

4.  Correlating telomerase activity levels with human neuroblastoma outcomes.

Authors:  E Hiyama; K Hiyama; T Yokoyama; Y Matsuura; M A Piatyszek; J W Shay
Journal:  Nat Med       Date:  1995-03       Impact factor: 53.440

5.  Specific association of human telomerase activity with immortal cells and cancer.

Authors:  N W Kim; M A Piatyszek; K R Prowse; C B Harley; M D West; P L Ho; G M Coviello; W E Wright; S L Weinrich; J W Shay
Journal:  Science       Date:  1994-12-23       Impact factor: 47.728
  5 in total
  169 in total

1.  Telomeric repeat amplification, without shortening or lengthening of the telomerase products: a method to analyze the processivity of telomerase enzyme.

Authors:  I Szatmari; J Aradi
Journal:  Nucleic Acids Res       Date:  2001-01-15       Impact factor: 16.971

2.  A novel functional genomics approach identifies mTERT as a suppressor of fibroblast transformation.

Authors:  Q X Li; J M Robbins; P J Welch; F Wong-Staal; J R Barber
Journal:  Nucleic Acids Res       Date:  2000-07-01       Impact factor: 16.971

3.  Cell senescence and telomere shortening induced by a new series of specific G-quadruplex DNA ligands.

Authors:  J F Riou; L Guittat; P Mailliet; A Laoui; E Renou; O Petitgenet; F Mégnin-Chanet; C Hélène; J L Mergny
Journal:  Proc Natl Acad Sci U S A       Date:  2002-02-19       Impact factor: 11.205

Review 4.  Complex regulation of telomerase activity: implications for cancer therapy.

Authors:  K S Elenitoba-Johnson
Journal:  Am J Pathol       Date:  2001-08       Impact factor: 4.307

5.  Rapid upregulation of telomerase activity in human leukemia HL-60 cells treated with clinical doses of the DNA-damaging drug etoposide.

Authors:  T J Moriarty; S Dupuis; C Autexier
Journal:  Leukemia       Date:  2002-06       Impact factor: 11.528

6.  Involvement of 14-3-3 proteins in nuclear localization of telomerase.

Authors:  H Seimiya; H Sawada; Y Muramatsu; M Shimizu; K Ohko; K Yamane; T Tsuruo
Journal:  EMBO J       Date:  2000-06-01       Impact factor: 11.598

7.  Constitutive and regulated expression of telomerase reverse transcriptase (hTERT) in human lymphocytes.

Authors:  K Liu; M M Schoonmaker; B L Levine; C H June; R J Hodes; N P Weng
Journal:  Proc Natl Acad Sci U S A       Date:  1999-04-27       Impact factor: 11.205

8.  C-terminal regions of the human telomerase catalytic subunit essential for in vivo enzyme activity.

Authors:  Soma S R Banik; Chuanhai Guo; Allyson C Smith; Seth S Margolis; D Ashley Richardson; Carlos A Tirado; Christopher M Counter
Journal:  Mol Cell Biol       Date:  2002-09       Impact factor: 4.272

9.  Non-canonical NF-κB signalling and ETS1/2 cooperatively drive C250T mutant TERT promoter activation.

Authors:  Yinghui Li; Qi-Ling Zhou; Wenjie Sun; Prashant Chandrasekharan; Hui Shan Cheng; Zhe Ying; Manikandan Lakshmanan; Anandhkumar Raju; Daniel G Tenen; Shi-Yuan Cheng; Kai-Hsiang Chuang; Jun Li; Shyam Prabhakar; Mengfeng Li; Vinay Tergaonkar
Journal:  Nat Cell Biol       Date:  2015-09-21       Impact factor: 28.824

10.  Telomere loss, senescence, and genetic instability in CD4+ T lymphocytes overexpressing hTERT.

Authors:  Alexander Röth; Gabriela M Baerlocher; Mike Schertzer; Elizabeth Chavez; Ulrich Dührsen; Peter M Lansdorp
Journal:  Blood       Date:  2005-03-01       Impact factor: 22.113
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