BACKGROUND: To clarify the pathological functions of the virulence factors of Helicobacter pylori, a comparative analysis was carried out on the relationship between motility, flagellar gene polymorphism, vacuolating cytotoxin (VT) production and interleukin-8 (IL-8) induction. METHODS: Twenty-five strains were examined for restriction fragment length polymorphism (RFLP) of the flagellin gene. Motility was measured using semisolid agar plates. Cytotoxicity was assayed using RK-13 cells. IL-8 secretion was assessed by the enzyme-linked immunosorbent assay (ELISA) methods. RESULTS: H. pylori was classified into four groups according to their flagellar RFLP. No differences were noted in motility or VT production among the four groups, but a significant difference was noted in IL-8 induction. In addition, highly motile strains produced more IL-8. CONCLUSION: This flagellar genetic polymorphism may be associated with IL-8 induction.
BACKGROUND: To clarify the pathological functions of the virulence factors of Helicobacter pylori, a comparative analysis was carried out on the relationship between motility, flagellar gene polymorphism, vacuolating cytotoxin (VT) production and interleukin-8 (IL-8) induction. METHODS: Twenty-five strains were examined for restriction fragment length polymorphism (RFLP) of the flagellin gene. Motility was measured using semisolid agar plates. Cytotoxicity was assayed using RK-13 cells. IL-8 secretion was assessed by the enzyme-linked immunosorbent assay (ELISA) methods. RESULTS:H. pylori was classified into four groups according to their flagellar RFLP. No differences were noted in motility or VT production among the four groups, but a significant difference was noted in IL-8 induction. In addition, highly motile strains produced more IL-8. CONCLUSION: This flagellar genetic polymorphism may be associated with IL-8 induction.
Authors: A Tasteyre; T Karjalainen; V Avesani; M Delmée; A Collignon; P Bourlioux; M C Barc Journal: J Clin Microbiol Date: 2001-03 Impact factor: 5.948
Authors: A Tasteyre; T Karjalainen; V Avesani; M Delmée; A Collignon; P Bourlioux; M C Barc Journal: J Clin Microbiol Date: 2000-09 Impact factor: 5.948
Authors: P Doig; B L de Jonge; R A Alm; E D Brown; M Uria-Nickelsen; B Noonan; S D Mills; P Tummino; G Carmel; B C Guild; D T Moir; G F Vovis; T J Trust Journal: Microbiol Mol Biol Rev Date: 1999-09 Impact factor: 11.056
Authors: Tonyia Eaves-Pyles; Heng-Fu Bu; Xiao-di Tan; Yingzi Cong; Jignesh Patel; Robert A Davey; Jane E Strasser Journal: PLoS One Date: 2011-09-15 Impact factor: 3.240