W S Ji1, J L Hu, J W Qiu, D R Peng, B L Shi, S J Zhou, K C Wu, D M Fan. 1. Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi'an 710032, Shaanxi Province, China. jiwsh@netease.com
Abstract
AIM: To study the polymorphism of flagellin A genotype and its significance in Helicobacter pylori (H. pylori). METHODS: As the template, genome DNA was purified from six clinical isolates of H. pylori from outpatients, and the corresponding flagellin A fragments were amplified by polymerase chain reaction. All these products were sequenced. These sequences were compared with each other, and analyzed by software of FASTA program. RESULTS: Specific PCR products were amplified from all of these H. pylori isolates and no length divergence was found among them. Compared with each other, the highest ungapped identity is 99.10%, while the lowest is 94.65%. Using FASTA program, the alignments between query and library sequences derived from different H. pylori strains were higher than 90%. CONCLUSION: The nucleotide sequence of flagellin A in H. pylori is highly conservative with incident divergence. This information may be useful for gene diagnosis and further study on flagellar antigen phenotype.
AIM: To study the polymorphism of flagellin A genotype and its significance in Helicobacter pylori (H. pylori). METHODS: As the template, genome DNA was purified from six clinical isolates of H. pylori from outpatients, and the corresponding flagellin A fragments were amplified by polymerase chain reaction. All these products were sequenced. These sequences were compared with each other, and analyzed by software of FASTA program. RESULTS: Specific PCR products were amplified from all of these H. pylori isolates and no length divergence was found among them. Compared with each other, the highest ungapped identity is 99.10%, while the lowest is 94.65%. Using FASTA program, the alignments between query and library sequences derived from different H. pylori strains were higher than 90%. CONCLUSION: The nucleotide sequence of flagellin A in H. pylori is highly conservative with incident divergence. This information may be useful for gene diagnosis and further study on flagellar antigen phenotype.
Authors: D Domingo; T Alarcón; J C Sanz; H Villar; J M Hernández; J Sánchez; M López-Brea Journal: Enferm Infecc Microbiol Clin Date: 1999 Aug-Sep Impact factor: 1.731
Authors: M A Monteiro; K H Chan; D A Rasko; D E Taylor; P Y Zheng; B J Appelmelk; H P Wirth; M Yang; M J Blaser; S O Hynes; A P Moran; M B Perry Journal: J Biol Chem Date: 1998-05-08 Impact factor: 5.157
Authors: R M Peek; M J Blaser; D J Mays; M H Forsyth; T L Cover; S Y Song; U Krishna; J A Pietenpol Journal: Cancer Res Date: 1999-12-15 Impact factor: 12.701