Literature DB >> 9172770

Specific antigen/antibody interactions measured by force microscopy.

U Dammer1, M Hegner, D Anselmetti, P Wagner, M Dreier, W Huber, H J Güntherodt.   

Abstract

Molecular recognition between biotinylated bovine serum albumin and polyclonal, biotin-directed IG antibodies has been measured directly under various buffer conditions using an atomic force microscope (AFM). It was found that even highly structured molecules such as IgG antibodies preserve their specific affinity to their antigens when probed with an AFM in the force mode. We could measure the rupture force between individual antibody-antigen complexes. The potential and limitations of this new approach for the measurement of individual antigen/antibody interactions and some possible applications are discussed.

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Year:  1996        PMID： 9172770      PMCID： PMC1225221          DOI： 10.1016/S0006-3495(96)79814-4

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  16 in total

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  70 in total

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10.  The effect of sedimentation and diffusion on cellular uptake of gold nanoparticles.

Authors:  Eun Chul Cho; Qiang Zhang; Younan Xia
Journal:  Nat Nanotechnol       Date:  2011-04-24       Impact factor: 39.213
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