Literature DB >> 9171435

Cloning, characterization, and sequence analysis of the clcE gene encoding the maleylacetate reductase of Pseudomonas sp. strain B13.

T Kasberg1, V Seibert, M Schlömann, W Reineke.   

Abstract

A 3,167-bp PstI fragment of genomic DNA from Pseudomonas sp. strain B13 was cloned and sequenced. The gene clcE consists of 1,059 nucleotides encoding a protein of 352 amino acids with a calculated mass of 37,769 Da which showed maleylacetate reductase activity. The protein had significant sequence similarities with the polypeptides encoded by tcbF of pP51 (59.4% identical positions), tfdF of pJP4 (55.1%), and tftE of Burkholderia cepacia AC1100 (53.1%). The function of TcbF as maleylacetate reductase was established by an enzyme assay.

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Year:  1997        PMID: 9171435      PMCID: PMC179183          DOI: 10.1128/jb.179.11.3801-3803.1997

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  17 in total

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Authors:  S R Kaschabek; W Reineke
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Authors:  S R Kaschabek; W Reineke
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Authors:  D L Daubaras; C D Hershberger; K Kitano; A M Chakrabarty
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  11 in total

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10.  Characterization of the maleylacetate reductase MacA of Rhodococcus opacus 1CP and evidence for the presence of an isofunctional enzyme.

Authors:  V Seibert; E M Kourbatova; L A Golovleva; M Schlömann
Journal:  J Bacteriol       Date:  1998-07       Impact factor: 3.490

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