| Literature DB >> 9171114 |
M Matz1, N Usman, D Shagin, E Bogdanova, S Lukyanov.
Abstract
A method for display of 3'-end restriction fragments of cDNAs is proposed, extending the idea reported recently. First, representative pools of such fragments are selectively amplified using PCR suppression effect. Then, simplified subsets of these fragments suitable for comparison by PAGE are amplified by adapter-specific primers extended by two randomly picked bases at their 3'ends. By testing all possible combinations of extended primers the whole mRNA pool may be systematically investigated. The method was applied to search for molecular regional markers of freshwater planarian Dugesia tigrina .Entities:
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Year: 1997 PMID: 9171114 PMCID: PMC146750 DOI: 10.1093/nar/25.12.2541
Source DB: PubMed Journal: Nucleic Acids Res ISSN: 0305-1048 Impact factor: 16.971