Literature DB >> 9819814

Differential display of mRNA.

J S Zhang1, E L Duncan, A C Chang, R R Reddel.   

Abstract

Differential display of mRNA (DD) is a technique in which mRNA species expressed by a cell population are reverse transcribed and then amplified by many separate polymerase chain reactions (PCR). PCR primers and conditions are chosen so that any given reaction yields a limited number of amplified cDNA fragments, permitting their visualization as discrete bands following gel electrophoresis. This robust and relatively simple procedure allows identification of genes that are differentially expressed in different cell populations. Here we review DD including some recent modifications, and compare it with other techniques for analyzing differential mRNA expression.

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Year:  1998        PMID： 9819814     DOI： 10.1007/BF02760862

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  59 in total

1.  Simulium damnosum s.l.: isolation and identification of prophenoloxidase following an infection with Onchocerca spp. using targeted differential display.

Authors:  H E Hagen; S L Kläger; J H McKerrow; P J Ham
Journal:  Exp Parasitol       Date:  1997-07       Impact factor: 2.011

2.  A model for p53-induced apoptosis.

Authors:  K Polyak; Y Xia; J L Zweier; K W Kinzler; B Vogelstein
Journal:  Nature       Date:  1997-09-18       Impact factor: 49.962

3.  Rational primer design greatly improves differential display-PCR (DD-PCR).

Authors:  D Graf; A G Fisher; M Merkenschlager
Journal:  Nucleic Acids Res       Date:  1997-06-01       Impact factor: 16.971

4.  Novel progesterone target genes identified by an improved differential display technique suggest that progestin-induced growth inhibition of breast cancer cells coincides with enhancement of differentiation.

Authors:  H A Kester; B M van der Leede; P T van der Saag; B van der Burg
Journal:  J Biol Chem       Date:  1997-06-27       Impact factor: 5.157

5.  Isolation of differentially expressed genes.

Authors:  T D Sargent
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

6.  Quantitative monitoring of gene expression patterns with a complementary DNA microarray.

Authors:  M Schena; D Shalon; R W Davis; P O Brown
Journal:  Science       Date:  1995-10-20       Impact factor: 47.728

7.  Differential display by PCR: novel findings and applications.

Authors:  M J Guimarães; F Lee; A Zlotnik; T McClanahan
Journal:  Nucleic Acids Res       Date:  1995-05-25       Impact factor: 16.971

8.  Non-radioisotopic differential display method to directly visualize and amplify differential bands on nylon membrane.

Authors:  J J Chen; K Peck
Journal:  Nucleic Acids Res       Date:  1996-02-15       Impact factor: 16.971

9.  Improvements to the differential display method for gene analysis.

Authors:  L Mou; H Miller; J Li; E Wang; L Chalifour
Journal:  Biochem Biophys Res Commun       Date:  1994-03-15       Impact factor: 3.575

10.  Identification by representational difference analysis of a homozygous deletion in pancreatic carcinoma that lies within the BRCA2 region.

Authors:  M Schutte; L T da Costa; S A Hahn; C Moskaluk; A T Hoque; E Rozenblum; C L Weinstein; M Bittner; P S Meltzer; J M Trent
Journal:  Proc Natl Acad Sci U S A       Date:  1995-06-20       Impact factor: 11.205
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  4 in total

Review 1.  Subtractive hybridization--genetic takeaways and the search for meaning.

Authors:  R J Byers; J A Hoyland; J Dixon; A J Freemont
Journal:  Int J Exp Pathol       Date:  2000-12       Impact factor: 1.925

2.  Identification of a reproductive-specific, putative lipid transport protein gene in a queenless ponerine ant Diacamma sp.

Authors:  Yasukazu Okada; Satoshi Miyazaki; Shigeyuki Koshikawa; Richard Cornette; Kiyoto Maekawa; Kazuki Tsuji; Toru Miura
Journal:  Naturwissenschaften       Date:  2010-09-25

Review 3.  Comprehensive gene expression analysis by transcript profiling.

Authors:  Jonathan Donson; Yiwen Fang; Gregg Espiritu-Santo; Weimei Xing; Andres Salazar; Susie Miyamoto; Veronica Armendarez; Wayne Volkmuth
Journal:  Plant Mol Biol       Date:  2002-01       Impact factor: 4.076

4.  From gene expression profiles to biological validation in enteroviral heart disease.

Authors:  Bobby Yanagawa; Bruce McManus; Zsuzsanna Hollander; Honglin Luo; Raymond Ng; Decheng Yang
Journal:  Exp Clin Cardiol       Date:  2003
  4 in total

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