N Hussain1, P U Jani, A T Florence. 1. Centre for Drug Delivery Research, School of Pharmacy, University of London, United Kingdom.
Abstract
PURPOSE: To investigate the usefulness of a surface-conjugated, bioadhesive molecule, tomato lectin, to augment intestinal uptake of orally administered inert nanoparticles. METHODS: Fluorescent 500 nm polystyrene nanoparticles with tomato lectin covalently surface coupled using a carbodiimide reaction were administered to female Wistar rats by oral gavage daily for 5 days. RESULTS: Analysis of tissue extracted polymer by gel permeation chromatography revealed a 23% systemic uptake of tomato lectin conjugated nanoparticles compared to < 0.5% of TL nanoparticles blocked with N-acetylchitotetraose thus representing an increase of almost 50 fold across the intestine. Intestinal uptake of tomato lectin-conjugated nanoparticles via the villous tissue was 15 times higher than uptake by the gut-associated lymphoid tissue. CONCLUSIONS: The application of tomato lectin as a bioadhesive agent in vivo has been demonstrated to enhance subsequent intestinal transcytosis of colloidal particulates to which it is bound.
PURPOSE: To investigate the usefulness of a surface-conjugated, bioadhesive molecule, tomatolectin, to augment intestinal uptake of orally administered inert nanoparticles. METHODS: Fluorescent 500 nm polystyrene nanoparticles with tomatolectin covalently surface coupled using a carbodiimide reaction were administered to female Wistar rats by oral gavage daily for 5 days. RESULTS: Analysis of tissue extracted polymer by gel permeation chromatography revealed a 23% systemic uptake of tomatolectin conjugated nanoparticles compared to < 0.5% of TL nanoparticles blocked with N-acetylchitotetraose thus representing an increase of almost 50 fold across the intestine. Intestinal uptake of tomatolectin-conjugated nanoparticles via the villous tissue was 15 times higher than uptake by the gut-associated lymphoid tissue. CONCLUSIONS: The application of tomatolectin as a bioadhesive agent in vivo has been demonstrated to enhance subsequent intestinal transcytosis of colloidal particulates to which it is bound.
Authors: Jwala Renukuntla; Aswani Dutt Vadlapudi; Ashaben Patel; Sai H S Boddu; Ashim K Mitra Journal: Int J Pharm Date: 2013-02-18 Impact factor: 5.875