Literature DB >> 9144799

Crystallization and preliminary X-ray analysis of Escherichia coli peptidyl-tRNA hydrolase.

E Schmitt1, M Fromant, P Plateau, Y Mechulam, S Blanquet.   

Abstract

Peptidyl-tRNA hydrolase from Escherichia coli, a monomer of 21 kDa, was overexpressed from its cloned gene pth and crystallized by using polyethylene glycol as precipitant. The crystals are orthorhombic and have unit cell parameters a = 47.24 A, b = 63.59 A, and c = 62.57 A. They belong to space group P2(1)2(1)2(1) and diffract to better than 1.2 A resolution. The structure is being solved by multiple isomorphous replacement.

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Year:  1997        PMID： 9144799     DOI： 10.1002/(sici)1097-0134(199705)28:1<135::aid-prot14>3.0.co;2-k

Source DB:  PubMed          Journal:  Proteins        ISSN： 0887-3585


  6 in total

1.  Crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Escherichia coli in complex with the acceptor-TΨC domain of tRNA.

Authors:  Kosuke Ito; Hao Qi; Yoshihiro Shimizu; Ryo Murakami; Kin-ichiro Miura; Takuya Ueda; Toshio Uchiumi
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2011-11-26

2.  RNA-binding site of Escherichia coli peptidyl-tRNA hydrolase.

Authors:  Laurent Giorgi; François Bontems; Michel Fromant; Caroline Aubard; Sylvain Blanquet; Pierre Plateau
Journal:  J Biol Chem       Date:  2011-09-19       Impact factor: 5.157

3.  Crystal structure at 1.2 A resolution and active site mapping of Escherichia coli peptidyl-tRNA hydrolase.

Authors:  E Schmitt; Y Mechulam; M Fromant; P Plateau; S Blanquet
Journal:  EMBO J       Date:  1997-08-01       Impact factor: 11.598

Review 4.  Bacterial transfer RNAs.

Authors:  Jennifer Shepherd; Michael Ibba
Journal:  FEMS Microbiol Rev       Date:  2015-03-21       Impact factor: 16.408

5.  Recombinant production, crystallization and X-ray crystallographic structure determination of the peptidyl-tRNA hydrolase of Pseudomonas aeruginosa.

Authors:  Ronny C Hughes; Hana McFeeters; Leighton Coates; Robert L McFeeters
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-11-28

6.  Small molecule binding, docking, and characterization of the interaction between Pth1 and peptidyl-tRNA.

Authors:  Mary C Hames; Hana McFeeters; W Blake Holloway; Christopher B Stanley; Volker S Urban; Robert L McFeeters
Journal:  Int J Mol Sci       Date:  2013-11-19       Impact factor: 5.923
  6 in total

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