Literature DB >> 9138562

Estimating the number of channels in patch-clamp recordings: application to kinetic analysis of multichannel data from voltage-operated channels.

W Baumgartner1, K Hohenthanner, G F Höfer, K Groschner, C Romanin.   

Abstract

Important kinetic information of voltage-operated ion channels can be obtained by estimating the open probability, the availability, and the first latency, and by applying run analysis. In the case of multichannel patches, estimation of the number of available channels is a prerequisite for the above analysis. Here we describe a method for calculation of the a posteriori probability of the number of available channels in each sweep by using the Bayes formula. This probability serves as a measure for the number of channels and allows for first latency determination and run analysis. The methods described were applied to simulated and experimental data obtained from L-type Ca2+ channel recordings.

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Year:  1997        PMID： 9138562      PMCID： PMC1184499          DOI： 10.1016/S0006-3495(97)78763-0

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  13 in total

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Authors:  F J Sigworth; J Zhou
Journal:  Methods Enzymol       Date:  1992       Impact factor: 1.600

2.  Characterization of single channel currents using digital signal processing techniques based on Hidden Markov Models.

Authors:  S H Chung; J B Moore; L G Xia; L S Premkumar; P W Gage
Journal:  Philos Trans R Soc Lond B Biol Sci       Date:  1990-09-29       Impact factor: 6.237

3.  Estimating the number of channels in patch recordings.

Authors:  R Horn
Journal:  Biophys J       Date:  1991-08       Impact factor: 4.033

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Authors:  L S Liebovitch; J Fischbarg
Journal:  Biochim Biophys Acta       Date:  1985-02-28

5.  On the stochastic properties of single ion channels.

Authors:  D Colquhoun; A G Hawkes
Journal:  Proc R Soc Lond B Biol Sci       Date:  1981-03-06

6.  Stable co-expression of calcium channel alpha 1, beta and alpha 2/delta subunits in a somatic cell line.

Authors:  A Welling; E Bosse; A Cavalié; R Bottlender; A Ludwig; W Nastainczyk; V Flockerzi; F Hofmann
Journal:  J Physiol       Date:  1993-11       Impact factor: 5.182

7.  Different modes of Ca channel gating behaviour favoured by dihydropyridine Ca agonists and antagonists.

Authors:  P Hess; J B Lansman; R W Tsien
Journal:  Nature       Date:  1984 Oct 11-17       Impact factor: 49.962

8.  A reinterpretation of mammalian sodium channel gating based on single channel recording.

Authors:  R W Aldrich; D P Corey; C F Stevens
Journal:  Nature       Date:  1983 Dec 1-7       Impact factor: 49.962

9.  A type 2A phosphatase-sensitive phosphorylation site controls modal gating of L-type Ca2+ channels in human vascular smooth-muscle cells.

Authors:  K Groschner; K Schuhmann; G Mieskes; W Baumgartner; C Romanin
Journal:  Biochem J       Date:  1996-09-01       Impact factor: 3.857

10.  Trypsin increases availability and open probability of cardiac L-type Ca2+ channels without affecting inactivation induced by Ca2+.

Authors:  R Schmid; K Seydl; W Baumgartner; K Groschner; C Romanin
Journal:  Biophys J       Date:  1995-11       Impact factor: 4.033
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  7 in total

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2.  Voltage-gated rearrangements associated with differential beta-subunit modulation of the L-type Ca(2+) channel inactivation.

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3.  Intracellular Ca2+ inhibits smooth muscle L-type Ca2+ channels by activation of protein phosphatase type 2B and by direct interaction with the channel.

Authors:  K Schuhmann; C Romanin; W Baumgartner; K Groschner
Journal:  J Gen Physiol       Date:  1997-11       Impact factor: 4.086

4.  Intracellular Ca2+ inactivates L-type Ca2+ channels with a Hill coefficient of approximately 1 and an inhibition constant of approximately 4 microM by reducing channel's open probability.

Authors:  G F Höfer; K Hohenthanner; W Baumgartner; K Groschner; N Klugbauer; F Hofmann; C Romanin
Journal:  Biophys J       Date:  1997-10       Impact factor: 4.033

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7.  Allosteric cross-talk between the hydrophobic cleft and the BH4 domain of Bcl-2 in control of inositol 1,4,5-trisphosphate receptor activity.

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