Literature DB >> 9136007

Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment.

D Cassidy-Hanley1, J Bowen, J H Lee, E Cole, L A VerPlank, J Gaertig, M A Gorovsky, P J Bruns.   

Abstract

Mating Tetrahymena thermophila were bombarded with ribosomal DNA-coated particles at various times in development. Both macronuclear and micronuclear transformants were recovered. Optimal developmental stages for transformation occurred during meiosis for the micronucleus and during anlagen formation for the macronucleus. Evidence is given for transient retention of the introduced plasmid. Genetic and molecular tests confirmed that sexually heritable transformation was associated with integration at the homologous site in the recipient micronuclear chromosome.

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Year:  1997        PMID： 9136007      PMCID： PMC1207932     

Source DB:  PubMed          Journal:  Genetics        ISSN： 0016-6731            Impact factor:   4.562


  20 in total

1.  A drug-resistant mutation in the ribosomal DNA of Tetrahymena.

Authors:  P J Bruns; A L Katzen; L Martin; E H Blackburn
Journal:  Proc Natl Acad Sci U S A       Date:  1985-05       Impact factor: 11.205

2.  Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes.

Authors:  M M Tondravi; M C Yao
Journal:  Proc Natl Acad Sci U S A       Date:  1986-06       Impact factor: 11.205

3.  Control of rDNA replication in Tetrahymena involves a cis-acting upstream repeat of a promoter element.

Authors:  D D Larson; E H Blackburn; P C Yaeger; E Orias
Journal:  Cell       Date:  1986-10-24       Impact factor: 41.582

4.  Transformation of Tetrahymena thermophila with hypermethylated rRNA genes.

Authors:  K M Karrer; M C Yao
Journal:  Mol Cell Biol       Date:  1988-04       Impact factor: 4.272

Review 5.  Genome organization and reorganization in Tetrahymena.

Authors:  M A Gorovsky
Journal:  Annu Rev Genet       Date:  1980       Impact factor: 16.830

6.  Genomic exclusion: a rapid means for inducing homozygous diploid lines in Tetrahymena pyriformis, syngen 1.

Authors:  S L Allen
Journal:  Science       Date:  1967-02-03       Impact factor: 47.728

7.  Cloning of abundant mRNA species present during conjugation of Tetrahymena thermophila: identification of mRNA species present exclusively during meiosis.

Authors:  D W Martindale; P J Bruns
Journal:  Mol Cell Biol       Date:  1983-10       Impact factor: 4.272

8.  A single integrated gene for ribosomal RNA in a eucaryote, Tetrahymena pyriformis.

Authors:  M C Yao; J G Gall
Journal:  Cell       Date:  1977-09       Impact factor: 41.582

9.  High frequency vector-mediated transformation and gene replacement in Tetrahymena.

Authors:  J Gaertig; L Gu; B Hai; M A Gorovsky
Journal:  Nucleic Acids Res       Date:  1994-12-11       Impact factor: 16.971

10.  Transformation of Tetrahymena thermophila by electroporation and parameters effecting cell survival.

Authors:  C F Brunk; P Navas
Journal:  Exp Cell Res       Date:  1988-02       Impact factor: 3.905
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  142 in total

1.  New class of cargo protein in Tetrahymena thermophila dense core secretory granules.

Authors:  Alex Haddad; Grant R Bowman; Aaron P Turkewitz
Journal:  Eukaryot Cell       Date:  2002-08

2.  Modulation of telomere length dynamics by the subtelomeric region of tetrahymena telomeres.

Authors:  Naduparambil K Jacob; Angela R Stout; Carolyn M Price
Journal:  Mol Biol Cell       Date:  2004-05-28       Impact factor: 4.138

3.  Conjugation-specific small RNAs in Tetrahymena have predicted properties of scan (scn) RNAs involved in genome rearrangement.

Authors:  Kazufumi Mochizuki; Martin A Gorovsky
Journal:  Genes Dev       Date:  2004-08-16       Impact factor: 11.361

4.  Zygotic expression of the double-stranded RNA binding motif protein Drb2p is required for DNA elimination in the ciliate Tetrahymena thermophila.

Authors:  Jason A Motl; Douglas L Chalker
Journal:  Eukaryot Cell       Date:  2011-10-21

5.  Constitutive expression, not a particular primary sequence, is the important feature of the H3 replacement variant hv2 in Tetrahymena thermophila.

Authors:  L Yu; M A Gorovsky
Journal:  Mol Cell Biol       Date:  1997-11       Impact factor: 4.272

6.  The CNA1 histone of the ciliate Tetrahymena thermophila is essential for chromosome segregation in the germline micronucleus.

Authors:  Marcella D Cervantes; Xiaohui Xi; Danielle Vermaak; Meng-Chao Yao; Harmit S Malik
Journal:  Mol Biol Cell       Date:  2005-10-26       Impact factor: 4.138

7.  Elimination of foreign DNA during somatic differentiation in Tetrahymena thermophila shows position effect and is dosage dependent.

Authors:  Yifan Liu; Xiaoyuan Song; Martin A Gorovsky; Kathleen M Karrer
Journal:  Eukaryot Cell       Date:  2005-02

8.  Unphosphorylated H1 is enriched in a specific region of the promoter when CDC2 is down-regulated during starvation.

Authors:  Xiaoyuan Song; Martin A Gorovsky
Journal:  Mol Cell Biol       Date:  2006-12-28       Impact factor: 4.272

9.  Centromeric histone H3 is essential for vegetative cell division and for DNA elimination during conjugation in Tetrahymena thermophila.

Authors:  Bowen Cui; Martin A Gorovsky
Journal:  Mol Cell Biol       Date:  2006-06       Impact factor: 4.272

10.  RNAi-dependent H3K27 methylation is required for heterochromatin formation and DNA elimination in Tetrahymena.

Authors:  Yifan Liu; Sean D Taverna; Tara L Muratore; Jeffrey Shabanowitz; Donald F Hunt; C David Allis
Journal:  Genes Dev       Date:  2007-06-15       Impact factor: 11.361
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