| Literature DB >> 9119087 |
N Kinto1, M Iwamoto, M Enomoto-Iwamoto, S Noji, H Ohuchi, H Yoshioka, H Kataoka, Y Wada, G Yuhao, H E Takahashi, S Yoshiki, A Yamaguchi.
Abstract
We investigated the role of Sonic hedgehog (SHH) in osteoblast differentiation and bone formation. The numbers of ALP-positive cells in the mouse fibroblastic cell line C3H10T1/2 and the mouse osteoblastic cell line MC3T3-E1 were increased by co-culture with chicken fibroblasts transfected with chicken Shh cDNA encoding amino-terminal peptide (Shh-N). The conditioned medium of Shh-N-RCAS-transfected chicken fibroblast cultures also significantly increased ALP activity in both C3H10T1/2 and MC3T3-E1 cells. Intramuscular transplantation of Shh-N-RCAS-transfected chicken fibroblasts into athymic mice induced ectopic bone formation. These results indicate that SHH induces osteoblast differentiation and ectopic bone formation.Entities:
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Year: 1997 PMID： 9119087 DOI： 10.1016/s0014-5793(97)00014-8
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124