| Literature DB >> 9114428 |
R A Respess1, A Butcher, H Wang, T Chaowanachan, N Young, N Shaffer, T D Mastro, B Biryahwaho, R Downing, A Tanuri, M Schechter, R Pascu, L Zekeng, L Kaptué, L Gürtler, J Eberle, D Ellenberger, C Fridlund, M Rayfield, S Kwok.
Abstract
A panel of 136 genetically diverse group M and 5 group O adult isolates from outside the United States and Europe were evaluated by PCR with the Roche AMPLICOR HIV-1 test, a modified version of the AMPLICOR HIV-1 test, and a new primer pair/probe system. Detection of some of these isolates was less efficient with the AMPLICOR HIV-1 test; however, the assay was significantly improved by reducing the sample input and lowering the annealing temperature. The new primer pair/probe set detected 140 of 141 isolates, including the 5 group O isolates that were not detected with either of the AMPLICOR HIV-1 test formats.Entities:
Mesh:
Year: 1997 PMID: 9114428 PMCID: PMC232750 DOI: 10.1128/jcm.35.5.1284-1286.1997
Source DB: PubMed Journal: J Clin Microbiol ISSN: 0095-1137 Impact factor: 5.948