Literature DB >> 10523553

Evaluation of the prototype Roche DNA amplification kit incorporating the new SSK145 and SKCC1B primers in detection of human immunodeficiency virus type 1 DNA in Zimbabwe.

L S Zijenah1, J Humphrey, K Nathoo, L Malaba, P Zvandasara, A Mahomva, P Iliff, M T Mbizvo.   

Abstract

We assessed the sensitivity and specificity of a newly developed DNA PCR kit (Roche Diagnostic Corporation, Indianapolis, Ind.) that incorporates primers for all the group M viruses for the detection of human immunodeficiency virus (HIV) type 1 (HIV-1) infection in Zimbabwe. A total of 202 whole-blood samples from adults whose HIV status was known were studied. This included 100 HIV-1-positive and 102 HIV-1-negative samples selected on the basis of concordant results obtained with two enzyme-linked immunosorbent assay kits. The prototype Roche DNA PCR assay had a 100% sensitivity for the detection of HIV-1 DNA and a specificity of 100%. We conclude that the new Roche DNA PCR kit is accurate for the detection of HIV DNA in Zimbabwean samples, in which HIV-1 subtype C dominates.

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Year:  1999        PMID： 10523553      PMCID： PMC85693     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  18 in total

1.  Polymerase chain reaction, virus isolation and antigen assay in HIV-1-antibody-positive mothers and their children.

Authors:  G Scarlatti; V Lombardi; A Plebani; N Principi; C Vegni; G Ferraris; A Bucceri; E M Fenyö; H Wigzell; P Rossi
Journal:  AIDS       Date:  1991-10       Impact factor: 4.177

2.  Comparison of HIV detection by virus isolation in lymphocyte cultures and molecular amplification of HIV DNA and RNA by PCR in offspring of seropositive mothers.

Authors:  S Escaich; M Wallon; I Baginski; J Ritter; N Philippe; Y Bertrand; O Claris; D Raudrant; M Sepetjan; C Trepo
Journal:  J Acquir Immune Defic Syndr (1988)       Date:  1991

3.  The polymerase chain reaction in the diagnosis of vertically transmitted HIV infection.

Authors:  P Williams; P Simmonds; P L Yap; P Balfe; J Bishop; R Brettle; R Hague; D Hargreaves; J Inglis; A L Brown
Journal:  AIDS       Date:  1990-05       Impact factor: 4.177

4.  Simplified and less expensive confirmatory HIV testing.

Authors:  G van der Groen; I Van Kerckhoven; G Vercauteren; P Piot
Journal:  Bull World Health Organ       Date:  1991       Impact factor: 9.408

5.  Polymerase chain reaction compared with concurrent viral cultures for rapid identification of human immunodeficiency virus infection among high-risk infants and children.

Authors:  J R Edwards; P P Ulrich; P S Weintrub; M J Cowan; J A Levy; D W Wara; G N Vyas
Journal:  J Pediatr       Date:  1989-08       Impact factor: 4.406

6.  Use of the polymerase chain reaction for early detection of the proviral sequences of human immunodeficiency virus in infants born to seropositive mothers. New York City Collaborative Study of Maternal HIV Transmission and Montefiore Medical Center HIV Perinatal Transmission Study Group.

Authors:  M F Rogers; C Y Ou; M Rayfield; P A Thomas; E E Schoenbaum; E Abrams; K Krasinski; P A Selwyn; J Moore; A Kaul
Journal:  N Engl J Med       Date:  1989-06-22       Impact factor: 91.245

7.  Genetic comparison of human immunodeficiency virus (HIV-1) isolates by polymerase chain reaction.

Authors:  F E McCutchan; E Sanders-Buell; C W Oster; R R Redfield; S K Hira; P L Perine; B L Ungar; D S Burke
Journal:  J Acquir Immune Defic Syndr (1988)       Date:  1991

8.  A comparative study of virus isolation, polymerase chain reaction, and antigen detection in children of mothers infected with human immunodeficiency virus.

Authors:  A Krivine; A Yakudima; M Le May; V Pena-Cruz; A S Huang; K McIntosh
Journal:  J Pediatr       Date:  1990-03       Impact factor: 4.406

9.  Early diagnosis of human immunodeficiency virus infection in children less than 6 months of age: comparison of polymerase chain reaction, culture, and plasma antigen capture techniques.

Authors:  W Borkowsky; K Krasinski; H Pollack; W Hoover; A Kaul; T Ilmet-Moore
Journal:  J Infect Dis       Date:  1992-09       Impact factor: 5.226

10.  Enzymatic amplification of the human immunodeficiency virus in peripheral blood mononuclear cells from pediatric patients.

Authors:  E G Chadwick; R Yogev; S Kwok; J J Sninsky; D E Kellogg; S M Wolinsky
Journal:  J Infect Dis       Date:  1989-12       Impact factor: 5.226
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  8 in total

1.  Evaluation of dried whole blood spots obtained by heel or finger stick as an alternative to venous blood for diagnosis of human immunodeficiency virus type 1 infection in vertically exposed infants in the routine diagnostic laboratory.

Authors:  Janet C Patton; Eveline Akkers; Ashraf H Coovadia; Tammy M Meyers; Wendy S Stevens; Gayle G Sherman
Journal:  Clin Vaccine Immunol       Date:  2006-12-13

2.  Clinical predictors of HIV infection in hospitalized children aged 2-18 months in Harare, Zimbabwe.

Authors:  K J Nathoo; S Rusakaniko; O Tobaiwa; H A Mujuru; I Ticklay; L Zijenah
Journal:  Afr Health Sci       Date:  2012-09       Impact factor: 0.927

3.  Antenatal HIV-1 RNA load and timing of mother to child transmission; a nested case-control study in a resource poor setting.

Authors:  Kerina Duri; Felicity Z Gumbo; Knut I Kristiansen; Nyaradzi E Kurewa; Munyaradzi P Mapingure; Simbarashe Rusakaniko; Mike Z Chirenje; Fredrik Muller; Babill Stray-Pedersen
Journal:  Virol J       Date:  2010-08-02       Impact factor: 4.099

4.  Performance of clinical algorithms for HIV-1 diagnosis and antiretroviral initiation among HIV-1-exposed children aged less than 18 months in Kenya.

Authors:  Irene Inwani; Dorothy Mbori-Ngacha; Ruth Nduati; Elizabeth Obimbo; Dalton Wamalwa; Grace John-Stewart; Carey Farquhar
Journal:  J Acquir Immune Defic Syndr       Date:  2009-04-15       Impact factor: 3.731

5.  Rapid, point-of-care extraction of human immunodeficiency virus type 1 proviral DNA from whole blood for detection by real-time PCR.

Authors:  Sujit R Jangam; Douglas H Yamada; Sally M McFall; David M Kelso
Journal:  J Clin Microbiol       Date:  2009-08       Impact factor: 5.948

6.  Simple DNA extraction method for dried blood spots and comparison of two PCR assays for diagnosis of vertical human immunodeficiency virus type 1 transmission in Rwanda.

Authors:  A Fischer; C Lejczak; C Lambert; J Servais; N Makombe; J Rusine; T Staub; R Hemmer; F Schneider; J C Schmit; V Arendt
Journal:  J Clin Microbiol       Date:  2004-01       Impact factor: 5.948

7.  Postmortem Study of Cause of Death Among Children Hospitalized With Respiratory Illness in Kenya.

Authors:  Henry N Njuguna; Sherif R Zaki; Drucilla J Roberts; Emily A Rogena; Edwin Walong; Corinne L Fligner; M Kelly Keating; Andrew K Gachii; Elizabeth Maleche-Obimbo; Grace Irimu; John Mathaiya; Noelle Orata; Rosemarie Lopokoiyit; Jackson Michuki; Gideon O Emukule; Clayton O Onyango; Stella Gikunju; Collins Owuor; Peter K Muturi; Milka Bunei; Maria Gloria Carvalho; Barry Fields; Joshua A Mott; Marc-Alain Widdowson; Sandra S Chaves
Journal:  Pediatr Infect Dis J       Date:  2021-08-01       Impact factor: 3.806

8.  T lymphocytes among HIV-infected and -uninfected infants: CD4/CD8 ratio as a potential tool in diagnosis of infection in infants under the age of 2 years.

Authors:  Lynn S Zijenah; David A Katzenstein; Kusum J Nathoo; Simbarashe Rusakaniko; Ocean Tobaiwa; Christine Gwanzura; Arsene Bikoue; Margaret Nhembe; Petronella Matibe; George Janossy
Journal:  J Transl Med       Date:  2005-02-01       Impact factor: 5.531
  8 in total

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