Literature DB >> 9114426

A rapid and simple method for extracting human immunodeficiency virus type 1 RNA from plasma: enhanced sensitivity.

J Mulder1, R Resnick, B Saget, S Scheibel, S Herman, H Payne, R Harrigan, S Kwok.   

Abstract

We present here a rapid and simple technique for processing human immunodeficiency virus (HIV)-infected plasma by high-speed centrifugation. HIV type 1 virions are pelleted from up to 1 ml of plasma, gently lysed with a nonionic detergent, and directly amplified. The procedure has few manipulations and requires approximately 1.5 h for the processing of 24 samples. Viral recovery ranges from 80 to 90%, with an analytical sensitivity approaching 20 copies/ml.

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Year:  1997        PMID： 9114426      PMCID： PMC232748          DOI： 10.1128/jcm.35.5.1278-1280.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


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10.  Use of multiple competitors for quantification of human immunodeficiency virus type 1 RNA in plasma.

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