Literature DB >> 9108161

A physico-chemical study of triple helix formation by an oligodeoxythymidylate with N3'--> P5' phosphoramidate linkages.

B Zhou-Sun1, J Sun, S M Gryaznov, J Liquier, T Garestier, C Hélène, E Taillandier.   

Abstract

Non-denaturing gel retardation assay, DNA melting experiments and FTIR spectroscopy were used to characterize the triple helix formed by a 15mer 2'-deoxythymidylate with N3'-->P5'phosphoramidate linkages with its target sequence. The results indicate that: (i) the pentadecadeoxythymidylate with phosphoramidate linkages [dT15(np)] is highly potent to form a triple helix with a dT15*dA15target duplex through Hoogsteenbase-pairing; (ii) it forms a dT15(np)*dA15xdT15(np) triplex with the single-stranded oligo-2'-deoxyadenylate (dA15) without detectable double-helical intermediate; (iii) it does not only form a triple helix on the dT15*dA15target duplex, but also partially displaces the dT15 strand from the dT15*dA15duplex to form a dT15(np)*dA15xdT15(np) complex.

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Year:  1997        PMID： 9108161      PMCID： PMC146641          DOI： 10.1093/nar/25.9.1782

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  15 in total

1.  Thermodynamics of triple helix formation: spectrophotometric studies on the d(A)10.2d(T)10 and d(C+3T4C+3).d(G3A4G3).d(C3T4C3) triple helices.

Authors:  D S Pilch; R Brousseau; R H Shafer
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

Review 2.  Specific regulation of gene expression by antisense, sense and antigene nucleic acids.

Authors:  C Hélène; J J Toulmé
Journal:  Biochim Biophys Acta       Date:  1990-06-21

3.  Structure and drug interactions of parallel-stranded DNA studied by infrared spectroscopy and fluorescence.

Authors:  H Fritzsche; A Akhebat; E Taillandier; K Rippe; T M Jovin
Journal:  Nucleic Acids Res       Date:  1993-11-11       Impact factor: 16.971

4.  Oligonucleotide interactions. 3. Circular dichroism studies of the conformation of deoxyoligonucleotides.

Authors:  C R Cantor; M M Warshaw; H Shapiro
Journal:  Biopolymers       Date:  1970       Impact factor: 2.505

Review 5.  Control of oncogene expression by antisense nucleic acids.

Authors:  C Hélène
Journal:  Eur J Cancer       Date:  1994       Impact factor: 9.162

Review 6.  Gene inhibition using antisense oligodeoxynucleotides.

Authors:  R W Wagner
Journal:  Nature       Date:  1994-11-24       Impact factor: 49.962

7.  Sequence-specific cleavage of double helical DNA by triple helix formation.

Authors:  H E Moser; P B Dervan
Journal:  Science       Date:  1987-10-30       Impact factor: 47.728

Review 8.  Antisense oligonucleotides as therapeutic agents--is the bullet really magical?

Authors:  C A Stein; Y C Cheng
Journal:  Science       Date:  1993-08-20       Impact factor: 47.728

9.  Selectivity of polyamines in triplex DNA stabilization.

Authors:  T Thomas; T J Thomas
Journal:  Biochemistry       Date:  1993-12-21       Impact factor: 3.162

10.  Triple helical polynucleotidic structures: sugar conformations determined by FTIR spectroscopy.

Authors:  J Liquier; P Coffinier; M Firon; E Taillandier
Journal:  J Biomol Struct Dyn       Date:  1991-12
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  2 in total

1.  Triplex targeting of human PDGF-B (c-sis, proto-oncogene) promoter specifically inhibits factors binding and PDGF-B transcription.

Authors:  J Liu; R Xu; Y Jin; D Wang
Journal:  Nucleic Acids Res       Date:  2001-02-01       Impact factor: 16.971

2.  Formation of stable triplexes between purine RNA and pyrimidine oligodeoxyxylonucleotides.

Authors:  Sergei Ivanov; Yakov Alekseev; Jean-Remi Bertrand; Claude Malvy; Marina B Gottikh
Journal:  Nucleic Acids Res       Date:  2003-07-15       Impact factor: 16.971
  2 in total

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