Literature DB >> 9092676

Improved efficiency for primer extension by using a long, highly-labeled primer generated from immobilized single-stranded DNA templates.

G Flouriot1, C Pope, M R Kenealy, F Gannon.   

Abstract

Primer extension is one of the most common methods used to measure the amount and size of RNAs. We demonstrate that the sensitivity and the specificity of this method are improved considerably by using a highly-labeled single-stranded DNA generated from a biotinylated single-stranded DNA template, as a long specific primer in the reverse transcription reaction. This new approach allows the detection of transcripts with a low expression level from microgram quantities of total RNA.

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Year:  1997        PMID： 9092676      PMCID： PMC146631          DOI： 10.1093/nar/25.8.1658

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  2 in total

1.  Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A.

Authors:  S Green; P Walter; V Kumar; A Krust; J M Bornert; P Argos; P Chambon
Journal:  Nature       Date:  1986 Mar 13-19       Impact factor: 49.962

2.  The genomic organisation, sequence and functional analysis of the 5' flanking region of the chicken estrogen receptor gene.

Authors:  P V Nestor; R C Forde; P Webb; F Gannon
Journal:  J Steroid Biochem Mol Biol       Date:  1994-08       Impact factor: 4.292
  2 in total
  1 in total

1.  Identification of differentially expressed 5'-end mRNA variants by an improved RACE technique (PEETA).

Authors:  G Flouriot; H Brand; F Gannon
Journal:  Nucleic Acids Res       Date:  1999-08-01       Impact factor: 16.971
  1 in total

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