Literature DB >> 9092646

A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution.

R K Bruick1, M Koppitz, G F Joyce, L E Orgel.   

Abstract

A rapid method for the synthesis of oligodeoxynucleotides (ODNs) terminated by 5'-amino-5'-deoxythymidine is described. A 3'-phosphorylated ODN (the donor) is incubated in aqueous solution with 5'-amino- 5'-deoxythymidine in the presence of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC), extending the donor by one residue via a phosphoramidate bond. Template- directed ligation of the extended donor and an acceptor ODN, followed by acid hydrolysis, yields the acceptor ODN extended by a single 5'-amino-5'-deoxythymidine residue at its 5'terminus.

Entities:  

Keywords:  NASA Discipline Exobiology; NASA Discipline Number 93-10; NASA Program NSCORT; Non-NASA Center

Mesh：

Substances：

Year:  1997        PMID： 9092646      PMCID： PMC146550          DOI： 10.1093/nar/25.6.1309

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  Preparation of oligonucleotide-peptide conjugates.

Authors:  C H Tung; M J Rudolph; S Stein
Journal:  Bioconjug Chem       Date:  1991 Nov-Dec       Impact factor: 4.774

2.  The synthesis and use of fluorescent oligonucleotides in DNA sequence analysis.

Authors:  L M Smith; R J Kaiser; J Z Sanders; L E Hood
Journal:  Methods Enzymol       Date:  1987       Impact factor: 1.600

3.  Phosphoramidate analogs of oligonucleotides.

Authors:  R L Letsinger; W S Mungall
Journal:  J Org Chem       Date:  1970-11       Impact factor: 4.354

4.  Derivatization of unprotected polynucleotides.

Authors:  B C Chu; G M Wahl; L E Orgel
Journal:  Nucleic Acids Res       Date:  1983-09-24       Impact factor: 16.971

5.  Incorporation of 5'-amino-5'-deoxythymidine5'-phosphate in polynucleotides by use of DNA polymerase I and a phiX174 DNA template.

Authors:  F L Letsinger; J S Wilkes
Journal:  Biochemistry       Date:  1976-06-29       Impact factor: 3.162

6.  Template-directed ligation of peptides to oligonucleotides.

Authors:  R K Bruick; P E Dawson; S B Kent; N Usman; G F Joyce
Journal:  Chem Biol       Date:  1996-01

7.  The synthesis of protected 5'-amino-2',5'-dideoxyribonucleoside-3'-O-phosphoramidites; applications of 5'-amino-oligodeoxyribonucleotides.

Authors:  B S Sproat; B Beijer; P Rider
Journal:  Nucleic Acids Res       Date:  1987-08-11       Impact factor: 16.971

8.  The synthesis of oligonucleotides containing an aliphatic amino group at the 5' terminus: synthesis of fluorescent DNA primers for use in DNA sequence analysis.

Authors:  L M Smith; S Fung; M W Hunkapiller; T J Hunkapiller; L E Hood
Journal:  Nucleic Acids Res       Date:  1985-04-11       Impact factor: 16.971
  8 in total

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