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Literature DB >> 1805945

Preparation of oligonucleotide-peptide conjugates.

Abstract

A procedure for preparing oligonucleotide-peptide conjugates is presented. It is based on appending a maleimide group to the oligonucleotide for selective coupling to the thiol side chain of a cysteine residue in the peptide. A convenient chromatographic purification procedure, based on Fmoc-on/Fmoc-off, is described.

Entities: Chemical

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Year: 1991 PMID： 1805945 DOI： 10.1021/bc00012a015

Source DB: PubMed Journal: Bioconjug Chem ISSN： 1043-1802 Impact factor: 4.774

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Cited

11 in total

1. A multilocus genotyping assay for candidate markers of cardiovascular disease risk.

Authors: S Cheng; M A Grow; C Pallaud; W Klitz; H A Erlich; S Visvikis; J J Chen; C R Pullinger; M J Malloy; G Siest; J P Kane
Journal: Genome Res Date: 1999-10 Impact factor: 9.043

2. Recurrent miscarriage and variant alleles of mannose binding lectin, tumour necrosis factor and lymphotoxin alpha genes.

Authors: N Baxter; M Sumiya; S Cheng; H Erlich; L Regan; A Simons; J A Summerfield
Journal: Clin Exp Immunol Date: 2001-12 Impact factor: 4.330

3. Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers.

Authors: K A Orle; C A Gates; D H Martin; B A Body; J B Weiss
Journal: J Clin Microbiol Date: 1996-01 Impact factor: 5.948

Preparation of oligonucleotide-peptide conjugates.

1. A multilocus genotyping assay for candidate markers of cardiovascular disease risk.

2. Recurrent miscarriage and variant alleles of mannose binding lectin, tumour necrosis factor and lymphotoxin alpha genes.

3. Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus types 1 and 2 from genital ulcers.

4. A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution.

5. Design of peptide-acridine mimics of ribonuclease activity.

6. Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction.

7. Polyamine-linked oligonucleotides for DNA triple helix formation.

8. An activated triple bond linker enables 'click' attachment of peptides to oligonucleotides on solid support.

Review 9. Exploiting protected maleimides to modify oligonucleotides, peptides and peptide nucleic acids.

10. Analysis of mRNA deadenylation by multi-protein complexes.