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Literature DB >> 9072182

Antigen retrieval, signal amplification and intensification in immunohistochemistry.

M Werner¹, R Von Wasielewski, P Komminoth.

Abstract

In this overview we emphasize new methods of improving immunohistochemical results in formaldehyde-fixed tissue samples. The benefit of heat-induced antigen retrieval in demasking of concealed epitopes is demonstrated. We provide guidance on the influence of heat-induced antigen retrieval in commonly applied monoclonal and polyclonal antibodies. Moreover, we show the promising methods of signal amplification using biotinylated tyramine and signal intensification of diaminobenzidine reaction products by metallic ions.

Entities: Chemical

Mesh：

Substances：

Year: 1996 PMID： 9072182 DOI： 10.1007/bf01463928

Source DB: PubMed Journal: Histochem Cell Biol ISSN： 0948-6143 Impact factor: 4.304

45 in total

1. The reaction of formaldehyde with proteins; participation of indole groups; gramicidin.

Authors: H FRAENKEL-CONRAT; B A BRANDON; H S OLCOTT
Journal: J Biol Chem Date: 1947-04 Impact factor: 5.157

Review 2. Assessment of antigen damage in immunohistochemistry. The vimentin internal control.

Authors: H Battifora
Journal: Am J Clin Pathol Date: 1991-11 Impact factor: 2.493

3. Reaction of formaldehyde with proteins; cross-linking of amino groups with phenol, imidazole, or indole groups.

Authors: H FRAENKEL-CONRAT; H S OLCOTT
Journal: J Biol Chem Date: 1948-07 Impact factor: 5.157

4. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.

Authors: S M Hsu; L Raine; H Fanger
Journal: J Histochem Cytochem Date: 1981-04 Impact factor: 2.479

5. Effect of fixation time and microwave oven heating time on retrieval of the Ki-67 antigen from paraffin-embedded tissue.

Authors: S Munakata; J B Hendricks
Journal: J Histochem Cytochem Date: 1993-08 Impact factor: 2.479

6. Antigen retrieval by microwave irradiation lowers immunohistological detection thresholds.

Authors: P H McKee; C Hobbs; P A Hall
Journal: Histopathology Date: 1993-10 Impact factor: 5.087

7. Heavy metal intensification of DAB-based HRP reaction product.

Authors: J C Adams
Journal: J Histochem Cytochem Date: 1981-06 Impact factor: 2.479

8. Alteration of immunoglobulin-bearing lymphoma cells by fixation.

Authors: R Warnke
Journal: J Histochem Cytochem Date: 1979-08 Impact factor: 2.479

9. Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays.

Authors: M N Bobrow; T D Harris; K J Shaughnessy; G J Litt
Journal: J Immunol Methods Date: 1989-12-20 Impact factor: 2.303

10. Overlapping expression of immunohistochemical markers and synaptophysin mRNA in pheochromocytomas and adrenocortical carcinomas. Implications for the differential diagnosis of adrenal gland tumors.

Authors: P Komminoth; J Roth; S Schröder; P Saremaslani; P U Heitz
Journal: Lab Invest Date: 1995-04 Impact factor: 5.662

28 in total

10. A simple and reliable pretreatment protocol facilitates fluorescent in situ hybridisation on tissue microarrays of paraffin wax embedded tumour samples.

Authors: S-F Chin; Y Daigo; H-E Huang; N G Iyer; G Callagy; T Kranjac; M Gonzalez; T Sangan; H Earl; C Caldas
Journal: Mol Pathol Date: 2003-10

Antigen retrieval, signal amplification and intensification in immunohistochemistry.

1. The reaction of formaldehyde with proteins; participation of indole groups; gramicidin.

Review 2. Assessment of antigen damage in immunohistochemistry. The vimentin internal control.

3. Reaction of formaldehyde with proteins; cross-linking of amino groups with phenol, imidazole, or indole groups.

4. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.

5. Effect of fixation time and microwave oven heating time on retrieval of the Ki-67 antigen from paraffin-embedded tissue.

6. Antigen retrieval by microwave irradiation lowers immunohistological detection thresholds.

7. Heavy metal intensification of DAB-based HRP reaction product.

8. Alteration of immunoglobulin-bearing lymphoma cells by fixation.

9. Catalyzed reporter deposition, a novel method of signal amplification. Application to immunoassays.

10. Overlapping expression of immunohistochemical markers and synaptophysin mRNA in pheochromocytomas and adrenocortical carcinomas. Implications for the differential diagnosis of adrenal gland tumors.

Review 1. Strategies for signal amplification in nucleic acid detection.

2. CARD In Situ Hybridization: Sights and Signals.

3. p27 cell-cycle inhibitor is inversely correlated with lymph node metastases in right-sided colon cancer.

Review 4. Applications of immunogold and lectin-gold labeling in tumor research and diagnosis.

5. CD44 standard and variant isoform expression in normal human skin appendages and epidermis.

6. CD44 isoform expression in the diffuse neuroendocrine system. I. Normal cells and hyperplasia.

Review 7. The role of histopathology in forensic practice: an overview.

8. Low level of cyclin D1 protein expression in thyroid microcarcinomas from an autopsy series.

9. Immunohistochemical markers for quantitative studies of neurons and glia in human neocortex.

10. A simple and reliable pretreatment protocol facilitates fluorescent in situ hybridisation on tissue microarrays of paraffin wax embedded tumour samples.