Literature DB >> 9055429

Molecular beacons: trial of a fluorescence-based solution hybridization technique for ecological studies with ruminal bacteria.

P Schofield1, A N Pell, D O Krause.   

Abstract

Molecular beacons are fluorescent probes developed for solution rather than membrane hybridization. We have investigated the utility of these probes to study rumen microbial ecology. Two cellulolytic species, Ruminococcus albus and Fibrobacter succinogenes, were tested. Membrane and solution hybridizations gave similar results in competition experiments with cocultures of R. albus 8 and F. succinogenes S85.

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Year:  1997        PMID： 9055429      PMCID： PMC168404          DOI： 10.1128/aem.63.3.1143-1147.1997

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  11 in total

Review 1.  DNA probes: applications of the principles of nucleic acid hybridization.

Authors:  J G Wetmur
Journal:  Crit Rev Biochem Mol Biol       Date:  1991       Impact factor: 8.250

2.  The anaerobic mesophilic cellulolytic bacteria.

Authors:  R E HUNGATE
Journal:  Bacteriol Rev       Date:  1950-03

3.  Ruminococcus flavefaciens Cell Coat and Adhesion to Cotton Cellulose and to Cell Walls in Leaves of Perennial Ryegrass (Lolium perenne).

Authors:  M J Latham; B E Brooker; G L Pettipher; P J Harris
Journal:  Appl Environ Microbiol       Date:  1978-01       Impact factor: 4.792

4.  Molecular beacons: probes that fluoresce upon hybridization.

Authors:  S Tyagi; F R Kramer
Journal:  Nat Biotechnol       Date:  1996-03       Impact factor: 54.908

5.  Commentary on the Hungate technique for culture of anaerobic bacteria.

Authors:  M P Bryant
Journal:  Am J Clin Nutr       Date:  1972-12       Impact factor: 7.045

6.  Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology.

Authors:  R I Amann; L Krumholz; D A Stahl
Journal:  J Bacteriol       Date:  1990-02       Impact factor: 3.490

7.  An rRNA approach for assessing the role of obligate amino acid-fermenting bacteria in ruminal amino acid deamination.

Authors:  D O Krause; J B Russell
Journal:  Appl Environ Microbiol       Date:  1996-03       Impact factor: 4.792

8.  Taxon-specific probes for the cellulolytic genus Fibrobacter reveal abundant and novel equine-associated populations.

Authors:  C Lin; D A Stahl
Journal:  Appl Environ Microbiol       Date:  1995-04       Impact factor: 4.792

9.  Use of phylogenetically based hybridization probes for studies of ruminal microbial ecology.

Authors:  D A Stahl; B Flesher; H R Mansfield; L Montgomery
Journal:  Appl Environ Microbiol       Date:  1988-05       Impact factor: 4.792

10.  The use of 16S rRNA-targeted oligonucleotide probes to study competition between ruminal fibrolytic bacteria: development of probes for Ruminococcus species and evidence for bacteriocin production.

Authors:  A A Odenyo; R I Mackie; D A Stahl; B A White
Journal:  Appl Environ Microbiol       Date:  1994-10       Impact factor: 4.792
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  4 in total

1.  Comparison of real-time, quantitative PCR with molecular beacons to nested PCR and culture methods for detection of Mycobacterium avium subsp. paratuberculosis in bovine fecal samples.

Authors:  Ying Fang; Wai-Hong Wu; Jessica L Pepper; Jill L Larsen; Salvatore A E Marras; Eric A Nelson; William B Epperson; Jane Christopher-Hennings
Journal:  J Clin Microbiol       Date:  2002-01       Impact factor: 5.948

2.  Use of DNA and peptide nucleic acid molecular beacons for detection and quantification of rRNA in solution and in whole cells.

Authors:  Chuanwu Xi; Michal Balberg; Stephen A Boppart; Lutgarde Raskin
Journal:  Appl Environ Microbiol       Date:  2003-09       Impact factor: 4.792

Review 3.  Detection and quantification of gene expression in environmental bacteriology.

Authors:  Freddie H Sharkey; Ibrahim M Banat; Roger Marchant
Journal:  Appl Environ Microbiol       Date:  2004-07       Impact factor: 4.792

4.  Application of a molecular beacon based real-time isothermal amplification (MBRTIA) technology for simultaneous detection of Bacillus cereus and Staphylococcus aureus.

Authors:  I M Mandappa; Prasanna Joglekar; H K Manonmani
Journal:  J Food Sci Technol       Date:  2014-08-20       Impact factor: 2.701
  4 in total

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