Literature DB >> 9041438

Evaluation of a quantitative plasma PCR plate assay for detecting cytomegalovirus infection in marrow transplant recipients.

G M Gallez-Hawkins1, B R Tegtmeier, A ter Veer, J C Niland, S J Forman, J A Zaia.   

Abstract

A plasma PCR test, using a nonradioactive PCR plate assay, was evaluated for detection of human cytomegalovirus reactivation. This assay was compared to Southern blotting and found to perform well. As a noncompetitive method of quantitation, it was similar to a competitive method for detecting the number of genome copies per milliliter of plasma in marrow transplant recipients. This is a technically simplified assay with potential for adaptation to automation.

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Year:  1997        PMID： 9041438      PMCID： PMC229676          DOI： 10.1128/jcm.35.3.788-790.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  23 in total

Review 1.  Treatment and prevention of cytomegalovirus pneumonia after bone marrow transplantation: where do we stand?

Authors:  S J Forman; J A Zaia
Journal:  Blood       Date:  1994-05-01       Impact factor: 22.113

2.  Cytomegalovirus excretion as a predictor of cytomegalovirus disease after marrow transplantation: importance of cytomegalovirus viremia.

Authors:  J D Meyers; P Ljungman; L D Fisher
Journal:  J Infect Dis       Date:  1990-08       Impact factor: 5.226

3.  Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction.

Authors:  G Gilliland; S Perrin; K Blanchard; H F Bunn
Journal:  Proc Natl Acad Sci U S A       Date:  1990-04       Impact factor: 11.205

4.  A randomized, controlled trial of prophylactic ganciclovir for cytomegalovirus pulmonary infection in recipients of allogeneic bone marrow transplants; The City of Hope-Stanford-Syntex CMV Study Group.

Authors:  G M Schmidt; D A Horak; J C Niland; S R Duncan; S J Forman; J A Zaia
Journal:  N Engl J Med       Date:  1991-04-11       Impact factor: 91.245

5.  Comparative quantitation of human cytomegalovirus DNA in blood leukocytes and plasma of transplant and AIDS patients.

Authors:  G Gerna; M Furione; F Baldanti; A Sarasini
Journal:  J Clin Microbiol       Date:  1994-11       Impact factor: 5.948

6.  Comparative analysis of human cytomegalovirus a-sequence in multiple clinical isolates by using polymerase chain reaction and restriction fragment length polymorphism assays.

Authors:  J A Zaia; G Gallez-Hawkins; M A Churchill; A Morton-Blackshere; H Pande; S P Adler; G M Schmidt; S J Forman
Journal:  J Clin Microbiol       Date:  1990-12       Impact factor: 5.948

7.  Quantitation of human cytomegalovirus (HCMV) DNA in cerebrospinal fluid by competitive PCR in AIDS patients with different HCMV central nervous system diseases.

Authors:  M Shinkai; S A Spector
Journal:  Scand J Infect Dis       Date:  1995

8.  Comparison of plasma PCR and bronchoalveolar lavage fluid culture for detection of cytomegalovirus infection in adult bone marrow transplant recipients.

Authors:  M M Aspin; G M Gallez-Hawkins; T D Giugni; B Tegtmeier; D J Lang; G M Schmidt; S J Forman; J A Zaia
Journal:  J Clin Microbiol       Date:  1994-09       Impact factor: 5.948

9.  Rapid immunodiagnosis of active cytomegalovirus infection by monoclonal antibody staining of blood leucocytes.

Authors:  W van der Bij; R Torensma; W J van Son; J Anema; J Schirm; A M Tegzess; T H The
Journal:  J Med Virol       Date:  1988-06       Impact factor: 2.327

10.  Quantitation of human immunodeficiency virus type 1 DNA and RNA by a novel internally controlled PCR assay.

Authors:  P Gupta; M Ding; M Cottrill; C Rinaldo; L Kingsley; S Wolinsky; J Mellors
Journal:  J Clin Microbiol       Date:  1995-06       Impact factor: 5.948
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  4 in total

1.  Enhanced analytical sensitivity of a quantitative PCR for CMV using a modified nucleic-acid extraction procedure.

Authors:  A Ferreira-Gonzalez; S Yanovich; M R Langley; L A Weymouth; D S Wilkinson; C T Garrett
Journal:  J Clin Lab Anal       Date:  2000       Impact factor: 2.352

2.  A highly sensitive assay for detection and quantitation of human cytomegalovirus DNA in serum and plasma by PCR and electrochemiluminescence.

Authors:  R Boom; C Sol; J Weel; Y Gerrits; M de Boer; P Wertheim-van Dillen
Journal:  J Clin Microbiol       Date:  1999-05       Impact factor: 5.948

3.  Infrequent occurrence of natural mutations in the pp65(495-503) epitope sequence presented by the HLA A*0201 allele among human cytomegalovirus isolates.

Authors:  J A Zaia; G Gallez-Hawkins; X Li; Z Q Yao; N Lomeli; K Molinder; C La Rosa; D J Diamond
Journal:  J Virol       Date:  2001-03       Impact factor: 5.103

4.  Evaluation of Murex CMV DNA hybrid capture assay for detection and quantitation of cytomegalovirus infection in patients following allogeneic stem cell transplantation.

Authors:  H Hebart; D Gamer; J Loeffler; C Mueller; C Sinzger; G Jahn; P Bader; T Klingebiel; L Kanz; H Einsele
Journal:  J Clin Microbiol       Date:  1998-05       Impact factor: 5.948
  4 in total

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