OBJECTIVE: Activation of both complement and neutrophils has been demonstrated to be involved in many pathological reactions following cardiopulmonary bypass (CPB). The aim of the present study is to evaluate the effect of normothermic and hypothermic CPB on both complement and neutrophil activation. METHODS: Two groups of patients (n = 20 each) scheduled for elective coronary artery bypass grafting, underwent CPB with intermittent warm or cold blood cardioplegia. Plasma concentration of C3a, C5a and C5b-9, as well as nitro-blu tetrazolium (NBT) scores of circulating neutrophils were measured before anesthesia, 10 and 30 min after the beginning of CPB, and 8, 16 and 24 h, postoperatively. RESULTS: In both groups, CPB determined a significant complement activation, evidenced as a significant increase in plasma concentration of C3a, C5a and C5b-9. This in turn triggered the neutrophil activation, documented as a significant increase of NTB scores in circulating neutrophils at the end of CPB and in the early postoperative period. Interestingly, in the warm group the extent of both complement and neutrophil activation was significantly higher compared with the cold group during the whole sampling period. CONCLUSION: In conclusion, our study clearly demonstrates that warm CPB is associated with an increased ability to activate complement and neutrophils in patients undergoing coronary surgery.
RCT Entities:
OBJECTIVE: Activation of both complement and neutrophils has been demonstrated to be involved in many pathological reactions following cardiopulmonary bypass (CPB). The aim of the present study is to evaluate the effect of normothermic and hypothermic CPB on both complement and neutrophil activation. METHODS: Two groups of patients (n = 20 each) scheduled for elective coronary artery bypass grafting, underwent CPB with intermittent warm or cold blood cardioplegia. Plasma concentration of C3a, C5a and C5b-9, as well as nitro-blu tetrazolium (NBT) scores of circulating neutrophils were measured before anesthesia, 10 and 30 min after the beginning of CPB, and 8, 16 and 24 h, postoperatively. RESULTS: In both groups, CPB determined a significant complement activation, evidenced as a significant increase in plasma concentration of C3a, C5a and C5b-9. This in turn triggered the neutrophil activation, documented as a significant increase of NTB scores in circulating neutrophils at the end of CPB and in the early postoperative period. Interestingly, in the warm group the extent of both complement and neutrophil activation was significantly higher compared with the cold group during the whole sampling period. CONCLUSION: In conclusion, our study clearly demonstrates that warm CPB is associated with an increased ability to activate complement and neutrophils in patients undergoing coronary surgery.
Authors: Hirotsugu Hamamoto; Hiroaki Sakamoto; Bradley G Leshnower; Landi M Parish; Shinya Kanemoto; Robin Hinmon; Theodore Plappert; Shinji Miyamoto; Martin G St John-Sutton; Joseph H Gorman; Robert C Gorman Journal: Ann Thorac Surg Date: 2009-01 Impact factor: 4.330
Authors: Shinya Kanemoto; Muneaki Matsubara; Mio Noma; Bradley G Leshnower; Landi M Parish; Benjamin M Jackson; Robin Hinmon; Hirotsugu Hamamoto; Joseph H Gorman; Robert C Gorman Journal: Ann Thorac Surg Date: 2009-01 Impact factor: 4.330
Authors: Thompson Ka Ming Kot; Jeffrey Shi Kai Chan; Saied Froghi; Dawnie Ho Hei Lau; Kara Morgan; Francesco Magni; Amer Harky Journal: JTCVS Open Date: 2021-03-31