C Garrett1, D Y Liu, H W Baker. 1. University of Melbourne, Department of Obstetrics and Gynecology, Victoria, Australia.
Abstract
OBJECTIVE: To obtain quantitative measures of morphometric selectivity of the human spermzona pellucida binding process as determined by light microscopy. DESIGN: Fully automated sperm head morphometric based on a 32-dimensional parameterization of images of Shorr-stained sperm. Zona pellucida selected sperm removed from reinseminated oocytes that previously failed IVF. SETTING: Academic research group associated with a tertiary infertility service. PATIENT(S): Semen samples from 51 infertile patients. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Differences in morphometric parameter means observed before and after swim-up and binding to zonae pellucidae. RESULT(S): Significant differences between insemination and bound sperm were observed in 17 parameter means and 21 standard deviations. The sperm-zona pellucida binding process preferentially selects sperm heads with a large anterior region with relatively low optical density, as well as high axial symmetry and minimal neck anomalies. Bias against sperm with pyriform morphology was not observed. CONCLUSION(S): A causal link has been established between sperm head morphometry, particularly within the acrosomal region, and the ability of sperm to bind to the human zona pellucida. As sperm-zona binding is necessary for fertility, it is possible to derive a physiologically based assessment for clinical diagnosis of male infertility using the "zona-preferred" morphometry results.
OBJECTIVE: To obtain quantitative measures of morphometric selectivity of the human spermzona pellucida binding process as determined by light microscopy. DESIGN: Fully automated sperm head morphometric based on a 32-dimensional parameterization of images of Shorr-stained sperm. Zona pellucida selected sperm removed from reinseminated oocytes that previously failed IVF. SETTING: Academic research group associated with a tertiary infertility service. PATIENT(S): Semen samples from 51 infertilepatients. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): Differences in morphometric parameter means observed before and after swim-up and binding to zonae pellucidae. RESULT(S): Significant differences between insemination and bound sperm were observed in 17 parameter means and 21 standard deviations. The sperm-zona pellucida binding process preferentially selects sperm heads with a large anterior region with relatively low optical density, as well as high axial symmetry and minimal neck anomalies. Bias against sperm with pyriform morphology was not observed. CONCLUSION(S): A causal link has been established between sperm head morphometry, particularly within the acrosomal region, and the ability of sperm to bind to the human zona pellucida. As sperm-zona binding is necessary for fertility, it is possible to derive a physiologically based assessment for clinical diagnosis of male infertility using the "zona-preferred" morphometry results.
Authors: Viviane Ribas Pereira; Danillo Roberto Pereira; Kátia Cristina de Melo Tavares Vieira; Vitor Pereira Ribas; Carlos José Leopoldo Constantino; Patrícia Alexandra Antunes; Ana Paula Alves Favareto Journal: Environ Sci Pollut Res Int Date: 2019-11-07 Impact factor: 4.223
Authors: Erica T Y Leung; Cheuk-Lun Lee; Xinyi Tian; Kevin K W Lam; Raymond H W Li; Ernest H Y Ng; William S B Yeung; Philip C N Chiu Journal: Nat Rev Urol Date: 2021-11-05 Impact factor: 14.432