Literature DB >> 9016772

Expression of Sulfolobus solfataricus trpE and trpG genes in E. coli.

M L Tutino1, A Tosco, G Marino, G Sannia.   

Abstract

The genes trpE and trpG of the hyperthermophilic archaeon Sulfolobus solfataricus, encoding the components I and II of anthranilate synthase, were cloned and co-expressed in Escherichia coli. The properties of the recombinant protein were determined and compared to those of the wild type complex. Gel filtration chromatography revealed an alpha2beta2 composition. The heteromeric enzyme is fully active above 85 degrees C and can be considered to be an "extremozyme" according to Adams et al.[1]. Sulfolobus solfataricus anthranilate synthase is subject to feedback inhibition by L-tryptophan even if it lacks the co-operativity that has been observed for all the other tetrameric anthranilate synthases.

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Year:  1997        PMID： 9016772     DOI： 10.1006/bbrc.1996.5951

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  7 in total

1.  Extrinsic factors potassium chloride and glycerol induce thermostability in recombinant anthranilate synthase from Archaeoglobus fulgidus.

Authors:  W Malcolm Byrnes; Vincent L Vilker
Journal:  Extremophiles       Date:  2004-07-02       Impact factor: 2.395

2.  The fused TrpEG from Streptomyces venezuelae is an anthranilate synthase, not a 2-amino-2-deoxyisochorismate [corrected] (ADIC) synthase.

Authors:  Meseret Ashenafi; Renee Carrington; Alvin C Collins; W Malcolm Byrnes
Journal:  Ethn Dis       Date:  2008       Impact factor: 1.847

3.  The crystal structure of anthranilate synthase from Sulfolobus solfataricus: functional implications.

Authors:  T Knöchel; A Ivens; G Hester; A Gonzalez; R Bauerle; M Wilmanns; K Kirschner; J N Jansonius
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-17       Impact factor: 11.205

4.  The fused anthranilate synthase from Streptomyces venezuelae functions as a monomer.

Authors:  Meseret Ashenafi; Prasad T Reddy; James F Parsons; W Malcolm Byrnes
Journal:  Mol Cell Biochem       Date:  2014-10-30       Impact factor: 3.396

5.  Novel stand-alone RAM domain protein-mediated catalytic control of anthranilate phosphoribosyltransferase in tryptophan biosynthesis in Thermus thermophilus.

Authors:  Tetsuo Kubota; Hajime Matsushita; Takeo Tomita; Saori Kosono; Minoru Yoshida; Tomohisa Kuzuyama; Makoto Nishiyama
Journal:  Extremophiles       Date:  2016-10-19       Impact factor: 2.395

6.  Two biosynthetic pathways for aromatic amino acids in the archaeon Methanococcus maripaludis.

Authors:  Iris Porat; Brian W Waters; Quincy Teng; William B Whitman
Journal:  J Bacteriol       Date:  2004-08       Impact factor: 3.490

7.  Architecture and functional dynamics of the pentafunctional AROM complex.

Authors:  Harshul Arora Verasztó; Maria Logotheti; Reinhard Albrecht; Alexander Leitner; Hongbo Zhu; Marcus D Hartmann
Journal:  Nat Chem Biol       Date:  2020-07-06       Impact factor: 15.040
  7 in total

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