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Literature DB >> 9016681

A method for high efficiency YAC lipofection into murine embryonic stem cells.

Abstract

We describe a modified protocol for introducing yeast artificial chromosomes (YACs) into murine embryonic stem (ES) cells by lipofection. With a decreased DNA:cell ratio, increased concentration of condensing agents and altered culture conditions, this protocol reduces the requirement for YAC DNA to a few micrograms, improves the recovery of neomycin-resistant ES colonies and increases the yield of clones containing both flanking vector markers and insert. These modifications enable generation of sufficient 'intact' transgenic clones for biological analysis with a single experiment.

Entities: Chemical Species

Mesh：

Year: 1996 PMID： 9016681 PMCID： PMC146328 DOI： 10.1093/nar/24.24.5054

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

9 in total

1. Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.

Authors: D T Burke; G F Carle; M V Olson
Journal: Science Date: 1987-05-15 Impact factor: 47.728

2. Mouse genetics. YACs to the rescue.

Authors: M R Capecchi
Journal: Nature Date: 1993-03-18 Impact factor: 49.962

Review 3. YAC transgenics and the study of genetics and human disease.

Authors: B T Lamb; J D Gearhart
Journal: Curr Opin Genet Dev Date: 1995-06 Impact factor: 5.578

4. Germ line transmission of a yeast artificial chromosome spanning the murine alpha 1(I) collagen locus.

Authors: W M Strauss; J Dausman; C Beard; C Johnson; J B Lawrence; R Jaenisch
Journal: Science Date: 1993-03-26 Impact factor: 47.728

5. De novo formation of several features of a centromere following introduction of a Y alphoid YAC into mammalian cells.

Authors: Z Larin; M D Fricker; C Tyler-Smith
Journal: Hum Mol Genet Date: 1994-05 Impact factor: 6.150

6. Introduction and expression of the 400 kilobase amyloid precursor protein gene in transgenic mice [corrected].

Authors: B T Lamb; S S Sisodia; A M Lawler; H H Slunt; C A Kitt; W G Kearns; P L Pearson; D L Price; J D Gearhart
Journal: Nat Genet Date: 1993-09 Impact factor: 38.330

7. A yeast artificial chromosome covering the tyrosinase gene confers copy number-dependent expression in transgenic mice.

Authors: A Schedl; L Montoliu; G Kelsey; G Schütz
Journal: Nature Date: 1993-03-18 Impact factor: 49.962

8. Transgenic mice containing a human heavy chain immunoglobulin gene fragment cloned in a yeast artificial chromosome.

Authors: T K Choi; P W Hollenbach; B E Pearson; R M Ueda; G N Weddell; C G Kurahara; C S Woodhouse; R M Kay; J F Loring
Journal: Nat Genet Date: 1993-06 Impact factor: 38.330

9. Antigen-specific human monoclonal antibodies from mice engineered with human Ig heavy and light chain YACs.

Authors: L L Green; M C Hardy; C E Maynard-Currie; H Tsuda; D M Louie; M J Mendez; H Abderrahim; M Noguchi; D H Smith; Y Zeng; N E David; H Sasai; D Garza; D G Brenner; J F Hales; R P McGuinness; D J Capon; S Klapholz; A Jakobovits
Journal: Nat Genet Date: 1994-05 Impact factor: 38.330