Literature DB >> 8348148

Transgenic mice containing a human heavy chain immunoglobulin gene fragment cloned in a yeast artificial chromosome.

T K Choi1, P W Hollenbach, B E Pearson, R M Ueda, G N Weddell, C G Kurahara, C S Woodhouse, R M Kay, J F Loring.   

Abstract

We have developed a method for the introduction of yeast artificial chromosomes (YACs) into transgenic mice. An 85 kilobase (kb) fragment of the human heavy chain immunoglobulin gene was cloned as a YAC, and embryonic stem cell lines carrying intact, integrated YACs were derived by co-lipofection of the YAC with an unlinked selectable marker. Chimaeric founder animals were produced by blastocyst injection, and offspring transgenic for the YAC were obtained. Analysis of serum from these offspring for human heavy chain antibody subunits demonstrated expression of the YAC-borne immunoglobulin gene fragment. Co-lipofection may prove to be a highly-successful means of producing transgenic mice containing large gene fragments in YACs.

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Year:  1993        PMID： 8348148     DOI： 10.1038/ng0693-117

Source DB:  PubMed          Journal:  Nat Genet        ISSN： 1061-4036            Impact factor:   38.330


  17 in total

Review 1.  Artificial chromosome-based transgenes in the study of genome function.

Authors:  Jason D Heaney; Sarah K Bronson
Journal:  Mamm Genome       Date:  2006-08-04       Impact factor: 2.957

2.  Transgene rescue in the mammary gland is associated with transcription but does not require translation of BLG transgenes.

Authors:  F Yull; B Binas; G Harold; R Wallace; A J Clark
Journal:  Transgenic Res       Date:  1997-01       Impact factor: 2.788

3.  A method for high efficiency YAC lipofection into murine embryonic stem cells.

Authors:  J T Lee; R Jaenisch
Journal:  Nucleic Acids Res       Date:  1996-12-15       Impact factor: 16.971

4.  Efficient male and female germline transmission of a human chromosomal vector in mice.

Authors:  T Voet; J Vermeesch; A Carens; J Dürr; C Labaere; H Duhamel; G David; P Marynen
Journal:  Genome Res       Date:  2001-01       Impact factor: 9.043

Review 5.  Size matters: use of YACs, BACs and PACs in transgenic animals.

Authors:  P Giraldo; L Montoliu
Journal:  Transgenic Res       Date:  2001-04       Impact factor: 2.788

6.  Neomycin- and hygromycin-resistance expression cassettes containing an artificial triple-helix site and a synthetic lac operator facilitate restriction endonuclease cleavage at pre-defined sites and recovery of specific fragments from mammalian genomes.

Authors:  M C Nehls; S Krause; T Boehm
Journal:  Mamm Genome       Date:  1994-03       Impact factor: 2.957

Review 7.  YAC transgenes: bigger is probably better.

Authors:  B G Forget
Journal:  Proc Natl Acad Sci U S A       Date:  1993-09-01       Impact factor: 11.205

Review 8.  Mapping the mouse genome: current status and future prospects.

Authors:  W F Dietrich; N G Copeland; D J Gilbert; J C Miller; N A Jenkins; E S Lander
Journal:  Proc Natl Acad Sci U S A       Date:  1995-11-21       Impact factor: 11.205

9.  Antibodies generated from human immunoglobulin miniloci in transgenic mice.

Authors:  S D Wagner; G T Williams; T Larson; M S Neuberger; D Kitamura; K Rajewsky; J Xian; M Brüggemann
Journal:  Nucleic Acids Res       Date:  1994-04-25       Impact factor: 16.971

10.  A method for the generation of YAC transgenic mice by pronuclear microinjection.

Authors:  A Schedl; Z Larin; L Montoliu; E Thies; G Kelsey; H Lehrach; G Schütz
Journal:  Nucleic Acids Res       Date:  1993-10-11       Impact factor: 16.971
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