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Literature DB >> 9016612

An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria.

J A Mangan¹, K M Sole, D A Mitchison, P D Butcher.

Abstract

A high yield, rapid and simple procedure is described for extracting RNA from mycobacteria and other micro-organisms refractory to disruption. The method yielded 20 microg RNA/109 Mycobacterium bovis BCG, more than 10 times greater than our previous method. Intact full length hsp 70 (dnaK) mRNA was detected by northern blotting and quantitated after heat shock by slot blot hybridisation.

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Year: 1997 PMID： 9016612 PMCID： PMC146465 DOI： 10.1093/nar/25.3.675

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

6 in total

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Authors: M D Bashyam; A Tyagi
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Review 5. Structure of mycobacteria: recent developments in defining cell wall carbohydrates and proteins.

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Journal: Rev Infect Dis Date: 1989 Mar-Apr

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