Literature DB >> 16081976

Approaches to bacterial RNA isolation and purification for microarray analysis of Escherichia coli K1 interaction with human brain microvascular endothelial cells.

Francescopaolo Di Cello1, Yi Xie, Maneesh Paul-Satyaseela, Kwang Sik Kim.   

Abstract

We established a protocol for isolation of microarray-grade bacterial RNA from Escherichia coli K1 interacting with human brain microvascular endothelial cells. The extracted RNA was free of human RNA contamination. More importantly, microarray analysis demonstrated that no bias was introduced in the gene expression pattern during the RNA isolation procedure.

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Year:  2005        PMID： 16081976      PMCID： PMC1233938          DOI： 10.1128/JCM.43.8.4197-4199.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


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