Literature DB >> 9008274

Molecular karyotype analysis of Cryptosporidium parvum: evidence for eight chromosomes and a low-molecular-size molecule.

D S Blunt1, N V Khramtsov, S J Upton, B A Montelone.   

Abstract

We report improved separation of chromosome-sized DNA molecules of the coccidian parasite Cryptosporidium parvum with contour-clamped homogeneous electric fields (CHEF). We used scanning densitometry to determine that the most likely number of chromosomes is eight. Molecular probes consisting of cloned genes were used to distinguish each of five bands visible on CHEF gels. We have also identified a low-molecular-size DNA molecule possibly related to the 35-kb circular DNAs found in other Apicomplexa.

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Year:  1997        PMID： 9008274      PMCID： PMC170468          DOI： 10.1128/cdli.4.1.11-13.1997

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  12 in total

1.  The putative acetyl-CoA synthetase gene of Cryptosporidium parvum and a new conserved protein motif in acetyl-CoA synthetases.

Authors:  N V Khramtsov; D S Blunt; B A Montelone; S J Upton
Journal:  J Parasitol       Date:  1996-06       Impact factor: 1.276

Review 2.  Pulsed field gel electrophoresis.

Authors:  E Lai; B W Birren; S M Clark; M I Simon; L Hood
Journal:  Biotechniques       Date:  1989-01       Impact factor: 1.993

Review 3.  The extrachromosomal DNAs of apicomplexan parasites.

Authors:  J E Feagin
Journal:  Annu Rev Microbiol       Date:  1994       Impact factor: 15.500

4.  DNA circles with cruciforms from Isospora (Toxoplasma) gondii.

Authors:  P Borst; J P Overdulve; P J Weijers; F Fase-Fowler; M Van den Berg
Journal:  Biochim Biophys Acta       Date:  1984-02-24

5.  Cloning and expression of a cDNA encoding epitopes shared by 15- and 60-kilodalton proteins of Cryptosporidium parvum sporozoites.

Authors:  M C Jenkins; R Fayer; M Tilley; S J Upton
Journal:  Infect Immun       Date:  1993-06       Impact factor: 3.441

6.  Isolation, sequence and molecular karyotype analysis of the actin gene of Cryptosporidium parvum.

Authors:  K Kim; L Goozé; C Petersen; J Gut; R G Nelson
Journal:  Mol Biochem Parasitol       Date:  1992-01       Impact factor: 1.759

7.  A Cryptosporidium parvum genomic region encoding hemolytic activity.

Authors:  M I Steele; T L Kuhls; K Nida; C S Meka; I M Halabi; D A Mosier; W Elliott; D L Crawford; R A Greenfield
Journal:  Infect Immun       Date:  1995-10       Impact factor: 3.441

8.  Characterization of a > 900,000-M(r) Cryptosporidium parvum sporozoite glycoprotein recognized by protective hyperimmune bovine colostral immunoglobulin.

Authors:  C Petersen; J Gut; P S Doyle; J H Crabb; R G Nelson; J H Leech
Journal:  Infect Immun       Date:  1992-12       Impact factor: 3.441

9.  Field inversion gel electrophoretic separation of Cryptosporidium spp. chromosome-sized DNA.

Authors:  J R Mead; M J Arrowood; W L Current; C R Sterling
Journal:  J Parasitol       Date:  1988-06       Impact factor: 1.276

10.  Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form Hsp70.

Authors:  N V Khramtsov; M Tilley; D S Blunt; B A Montelone; S J Upton
Journal:  J Eukaryot Microbiol       Date:  1995 Jul-Aug       Impact factor: 3.346
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  10 in total

1.  A HAPPY map of Cryptosporidium parvum.

Authors:  M B Piper; A T Bankier; P H Dear
Journal:  Genome Res       Date:  1998-12       Impact factor: 9.043

Review 2.  Genomics of apicomplexan parasites.

Authors:  Lakshmipuram Seshadri Swapna; John Parkinson
Journal:  Crit Rev Biochem Mol Biol       Date:  2017-02-22       Impact factor: 8.250

Review 3.  Cryptosporidium pathogenicity and virulence.

Authors:  Maha Bouzid; Paul R Hunter; Rachel M Chalmers; Kevin M Tyler
Journal:  Clin Microbiol Rev       Date:  2013-01       Impact factor: 26.132

4.  Extensive polymorphism in Cryptosporidium parvum identified by multilocus microsatellite analysis.

Authors:  X Feng; S M Rich; D Akiyoshi; J K Tumwine; A Kekitiinwa; N Nabukeera; S Tzipori; G Widmer
Journal:  Appl Environ Microbiol       Date:  2000-08       Impact factor: 4.792

5.  A random survey of the Cryptosporidium parvum genome.

Authors:  C Liu; V Vigdorovich; V Kapur; M S Abrahamsen
Journal:  Infect Immun       Date:  1999-08       Impact factor: 3.441

Review 6.  New insights into human cryptosporidiosis.

Authors:  D P Clark
Journal:  Clin Microbiol Rev       Date:  1999-10       Impact factor: 26.132

7.  Integrated mapping, chromosomal sequencing and sequence analysis of Cryptosporidium parvum.

Authors:  Alan T Bankier; Helen F Spriggs; Berthold Fartmann; Bernard A Konfortov; Martin Madera; Christine Vogel; Sarah A Teichmann; Al Ivens; Paul H Dear
Journal:  Genome Res       Date:  2003-07-17       Impact factor: 9.043

8.  Multilocus genotypic analysis of Cryptosporidium parvum isolates from different hosts and geographical origins.

Authors:  F Spano; L Putignani; A Crisanti; P Sallicandro; U M Morgan; S M Le Blancq; L Tchack; S Tzipori; G Widmer
Journal:  J Clin Microbiol       Date:  1998-11       Impact factor: 5.948

9.  Nuclear-encoded proteins target to the plastid in Toxoplasma gondii and Plasmodium falciparum.

Authors:  R F Waller; P J Keeling; R G Donald; B Striepen; E Handman; N Lang-Unnasch; A F Cowman; G S Besra; D S Roos; G I McFadden
Journal:  Proc Natl Acad Sci U S A       Date:  1998-10-13       Impact factor: 11.205

10.  Cryptosporidium parvum genome project.

Authors:  M S Abrahamsen
Journal:  Comp Funct Genomics       Date:  2001
  10 in total

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