Literature DB >> 3288738

Field inversion gel electrophoretic separation of Cryptosporidium spp. chromosome-sized DNA.

J R Mead1, M J Arrowood, W L Current, C R Sterling.   

Abstract

Chromosomal DNA from 5 isolates of Cryptosporidium parvum and 1 of C. baileyi were compared by field-inversion gel electrophoresis (FIGE). FIGE analyses of parasite DNA prepared from purified sporozoites versus intact oocysts showed no observable differences. Chromosomal DNA migration patterns of the 5 C. parvum isolates were indistinguishable, whereas similar but distinct differences were evident between C. baileyi and the isolates of C. parvum. Oocyst-reactive monoclonal antibodies differentiated oocysts of C. parvum from those of C. baileyi but were unable to distinguish oocysts of 1 isolate of C. parvum from another.

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Year:  1988        PMID： 3288738

Source DB:  PubMed          Journal:  J Parasitol        ISSN： 0022-3395            Impact factor:   1.276


  18 in total

1.  Histopathological method for diagnosis of cryptosporidiosis using monoclonal antibodies.

Authors:  A Bonnin; T Petrella; J F Dubremetz; J F Michiels; D Puygauthier-Toubas; P Camerlynck
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1990-09       Impact factor: 3.267

Review 2.  A hundred-year retrospective on cryptosporidiosis.

Authors:  Saul Tzipori; Giovanni Widmer
Journal:  Trends Parasitol       Date:  2008-03-07

Review 3.  Epidemiological aspects of human cryptosporidiosis.

Authors:  D P Casemore
Journal:  Epidemiol Infect       Date:  1990-02       Impact factor: 2.451

4.  A HAPPY map of Cryptosporidium parvum.

Authors:  M B Piper; A T Bankier; P H Dear
Journal:  Genome Res       Date:  1998-12       Impact factor: 9.043

5.  Molecular karyotype analysis of Cryptosporidium parvum: evidence for eight chromosomes and a low-molecular-size molecule.

Authors:  D S Blunt; N V Khramtsov; S J Upton; B A Montelone
Journal:  Clin Diagn Lab Immunol       Date:  1997-01

6.  Identification of isolate-specific sporozoite proteins of Cryptosporidium parvum by two-dimensional gel electrophoresis.

Authors:  J R Mead; R C Humphreys; D W Sammons; C R Sterling
Journal:  Infect Immun       Date:  1990-07       Impact factor: 3.441

7.  The genome of Eimeria tenella: further studies on its molecular organisation.

Authors:  M W Shirley
Journal:  Parasitol Res       Date:  1994       Impact factor: 2.289

8.  Analysis of oocyst wall and sporozoite antigens from three Cryptosporidium species.

Authors:  J M Nina; V McDonald; D A Dyson; J Catchpole; S Uni; M Iseki; P L Chiodini; K P McAdam
Journal:  Infect Immun       Date:  1992-04       Impact factor: 3.441

9.  A Cryptosporidium parvum genomic region encoding hemolytic activity.

Authors:  M I Steele; T L Kuhls; K Nida; C S Meka; I M Halabi; D A Mosier; W Elliott; D L Crawford; R A Greenfield
Journal:  Infect Immun       Date:  1995-10       Impact factor: 3.441

10.  Neutralization-sensitive epitopes are conserved among geographically diverse isolates of Cryptosporidium parvum.

Authors:  E W Uhl; R M O'Connor; L E Perryman; M W Riggs
Journal:  Infect Immun       Date:  1992-04       Impact factor: 3.441
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