Literature DB >> 9007639

An improved method for MN genotyping by the polymerase chain reaction.

N Nakayashiki1, Y Sasaki.   

Abstract

A novel method of human MN blood group genotyping is reported using the polymerase chain reaction. Genotyping is based on two base substitutions characteristic of M and N alleles in the 2nd exon of the glycophorin A gene. Using a newly designed primer trio, PCR products for M (255 bp) and N (270 bp) alleles are rapidly and simultaneously detected by a single PCR procedure and subsequent polyacrylamide gel electrophoresis. This method enables MN genotyping from not only minute but also degraded DNA samples.

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Year:  1996        PMID： 9007639     DOI： 10.1007/bf01225522

Source DB:  PubMed          Journal:  Int J Legal Med        ISSN： 0937-9827            Impact factor:   2.686


  4 in total

1.  Polymerase chain reaction-based detection of MN blood group-specific sequences in the human genome.

Authors:  V A Corfield; J C Moolman; R Martell; P A Brink
Journal:  Transfusion       Date:  1993-02       Impact factor: 3.157

2.  Molecular genetics of human erythrocyte MiIII and MiVI glycophorins. Use of a pseudoexon in construction of two delta-alpha-delta hybrid genes resulting in antigenic diversification.

Authors:  C H Huang; O O Blumenfeld
Journal:  J Biol Chem       Date:  1991-04-15       Impact factor: 5.157

3.  Molecular cloning of a human glycophorin B cDNA: nucleotide sequence and genomic relationship to glycophorin A.

Authors:  P D Siebert; M Fukuda
Journal:  Proc Natl Acad Sci U S A       Date:  1987-10       Impact factor: 11.205

4.  Isolation and characterization of human glycophorin A cDNA clones by a synthetic oligonucleotide approach: nucleotide sequence and mRNA structure.

Authors:  P D Siebert; M Fukuda
Journal:  Proc Natl Acad Sci U S A       Date:  1986-03       Impact factor: 11.205
  4 in total
  2 in total

1.  Sequence-specific primers for MNS blood group genotyping.

Authors:  Guido A Heymann; Abdulgabar Salama
Journal:  Blood Transfus       Date:  2010-07       Impact factor: 3.443

2.  Systematic classification of alleles of the glycophorin A (MN blood group) gene.

Authors:  Hajime Mizukami; Atsushi Akane; Nori Nakayashiki; Yasuhiro Aoki; Hiroshi Shiono
Journal:  J Hum Genet       Date:  2005-10-05       Impact factor: 3.172
  2 in total

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