Literature DB >> 9006057

Characterization of the intergenic region which regulates the MspI restriction-modification system.

S Som1, S Friedman.   

Abstract

The 110-bp intergenic region between mspIM and mspIR, the genes encoding the MspI modification (M.MspI) and restriction (R.MspI) enzymes, respectively, was fused, in both orientations, with lacZ. Expression of a single-copy mspIM-lacZ fusion is more than 400-fold stronger than expression of an mspIR-lacZ fusion. M.MspI in trans represses expression of the mspIM-lacZ fusion by binding to the DNA but does not affect expression of the mspIR-lacZ fusion. Transcription start sites of the genes were identified, and a set of nonoverlapping promoters was assigned. DNase I footprinting showed that M.MspI binds to a site within the intergenic region that includes only the mspIM regulatory elements.

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Year:  1997        PMID： 9006057      PMCID： PMC178784          DOI： 10.1128/jb.179.3.964-967.1997

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  15 in total

Review 1.  Organization of restriction-modification systems.

Authors:  G G Wilson
Journal:  Nucleic Acids Res       Date:  1991-05-25       Impact factor: 16.971

2.  Purification and characterization of the MspI DNA methyltransferase cloned and overexpressed in E. coli.

Authors:  A K Dubey; B Mollet; R J Roberts
Journal:  Nucleic Acids Res       Date:  1992-04-11       Impact factor: 16.971

3.  A family of regulatory genes associated with type II restriction-modification systems.

Authors:  T Tao; J C Bourne; R M Blumenthal
Journal:  J Bacteriol       Date:  1991-02       Impact factor: 3.490

4.  Cloning and characterization of the genes encoding the MspI restriction modification system.

Authors:  P M Lin; C H Lee; R J Roberts
Journal:  Nucleic Acids Res       Date:  1989-04-25       Impact factor: 16.971

5.  Analysis of E. coli promoter sequences.

Authors:  C B Harley; R P Reynolds
Journal:  Nucleic Acids Res       Date:  1987-03-11       Impact factor: 16.971

6.  Improved single and multicopy lac-based cloning vectors for protein and operon fusions.

Authors:  R W Simons; F Houman; N Kleckner
Journal:  Gene       Date:  1987       Impact factor: 3.688

7.  Regulation of EcoRII methyltransferase: effect of mutations on gene expression and in vitro binding to the promoter region.

Authors:  S Som; S Friedman
Journal:  Nucleic Acids Res       Date:  1994-12-11       Impact factor: 16.971

8.  Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors:  A M Maxam; W Gilbert
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600

9.  Cloning and expression of the MspI restriction and modification genes.

Authors:  D O Nwankwo; G G Wilson
Journal:  Gene       Date:  1988-04-15       Impact factor: 3.688

10.  The amino acid sequence of the CCGG recognizing DNA methyltransferase M.BsuFI: implications for the analysis of sequence recognition by cytosine DNA methyltransferases.

Authors:  J Walter; M Noyer-Weidner; T A Trautner
Journal:  EMBO J       Date:  1990-04       Impact factor: 11.598
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  11 in total

1.  Transcriptional analysis and regulation of expression of the ScrFI restriction-modification system of Lactococcus lactis subsp. cremoris UC503.

Authors:  D Butler; G F Fitzgerald
Journal:  J Bacteriol       Date:  2001-08       Impact factor: 3.490

2.  DNA methylation at the CfrBI site is involved in expression control in the CfrBI restriction-modification system.

Authors:  I V Beletskaya; M V Zakharova; M G Shlyapnikov; L M Semenova; A S Solonin
Journal:  Nucleic Acids Res       Date:  2000-10-01       Impact factor: 16.971

3.  The methyltransferase from the LlaDII restriction-modification system influences the level of expression of its own gene.

Authors:  Lisa Lystbaek Christensen; Jytte Josephsen
Journal:  J Bacteriol       Date:  2004-01       Impact factor: 3.490

Review 4.  [Regulation of gene expression in type II restriction-modification system].

Authors:  M O Nagornykh; E S Bogdanova; A S Protsenko; M V Zakharova; A S Solonin; K V Severinov
Journal:  Genetika       Date:  2008-05

5.  DNA-methyltransferase SsoII interaction with own promoter region binding site.

Authors:  I Shilov; V Tashlitsky; M Khodoun; S Vasil'ev; Y Alekseev; A Kuzubov; E Kubareva; A Karyagina
Journal:  Nucleic Acids Res       Date:  1998-06-01       Impact factor: 16.971

6.  Transcription regulation of restriction-modification system Ecl18kI.

Authors:  Alexey Protsenko; Marina Zakharova; Maxim Nagornykh; Alexander Solonin; Konstantin Severinov
Journal:  Nucleic Acids Res       Date:  2009-07-10       Impact factor: 16.971

7.  Controller protein of restriction-modification system Kpn2I affects transcription of its gene by acting as a transcription elongation roadblock.

Authors:  Evgeny Klimuk; Ekaterina Bogdanova; Max Nagornykh; Andjela Rodic; Marko Djordjevic; Sofia Medvedeva; Olga Pavlova; Konstantin Severinov
Journal:  Nucleic Acids Res       Date:  2018-11-16       Impact factor: 16.971

8.  Antisense RNA associated with biological regulation of a restriction-modification system.

Authors:  Iwona Mruk; Yaoping Liu; Liying Ge; Ichizo Kobayashi
Journal:  Nucleic Acids Res       Date:  2011-03-31       Impact factor: 16.971

9.  Flexibility of the linker between the domains of DNA methyltransferase SsoII revealed by small-angle X-ray scattering: implications for transcription regulation in SsoII restriction-modification system.

Authors:  Petr V Konarev; Galina S Kachalova; Alexandra Yu Ryazanova; Elena A Kubareva; Anna S Karyagina; Hans D Bartunik; Dmitri I Svergun
Journal:  PLoS One       Date:  2014-04-07       Impact factor: 3.240

Review 10.  To be or not to be: regulation of restriction-modification systems and other toxin-antitoxin systems.

Authors:  Iwona Mruk; Ichizo Kobayashi
Journal:  Nucleic Acids Res       Date:  2013-08-13       Impact factor: 16.971
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