Literature DB >> 9003626

A modified Elek test for detection of toxigenic corynebacteria in the diagnostic laboratory.

K H Engler1, T Glushkevich, I K Mazurova, R C George, A Efstratiou.   

Abstract

The detection of toxigenicity among Corynebacterium diphtheriae and Corynebacterium ulcerans strains is the most important test for the microbiological diagnosis of diphtheria. Difficulties with current methods, in particular the Elek test, are well documented. We therefore describe a modified Elek test which provides an accurate result after only 16 h of incubation, in contrast to 48 h for the conventional test.

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Year:  1997        PMID： 9003626      PMCID： PMC229610          DOI： 10.1128/jcm.35.2.495-498.1997

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  7 in total

1.  Screening tests for pathogenic corynebacteria.

Authors:  G Colman; E Weaver; A Efstratiou
Journal:  J Clin Pathol       Date:  1992-01       Impact factor: 3.411

Review 2.  Current situation and control strategies for resurgence of diphtheria in newly independent states of the former Soviet Union.

Authors:  I R Hardy; S Dittmann; R W Sutter
Journal:  Lancet       Date:  1996-06-22       Impact factor: 79.321

3.  Polymerase chain reaction assay for diagnosis of potentially toxinogenic Corynebacterium diphtheriae strains: correlation with ADP-ribosylation activity assay.

Authors:  D Hauser; M R Popoff; M Kiredjian; P Boquet; F Bimet
Journal:  J Clin Microbiol       Date:  1993-10       Impact factor: 5.948

4.  Application of PCR for detection of toxigenic Corynebacterium diphtheriae strains isolated during the Russian diphtheria epidemic, 1990 through 1994.

Authors:  V M Mikhailovich; V G Melnikov; I K Mazurova; I K Wachsmuth; J D Wenger; M Wharton; H Nakao; T Popovic
Journal:  J Clin Microbiol       Date:  1995-11       Impact factor: 5.948

5.  Polymerase chain reaction for screening clinical isolates of corynebacteria for the production of diphtheria toxin.

Authors:  M J Pallen; A J Hay; L H Puckey; A Efstratiou
Journal:  J Clin Pathol       Date:  1994-04       Impact factor: 3.411

6.  Detection of toxin production by Corynebacterium diphtheriae: results of a trial organised as part of the United Kingdom National External Microbiological Quality Assessment Scheme.

Authors:  J J Snell; J V Demello; P S Gardner; W Kwantes; R Brooks
Journal:  J Clin Pathol       Date:  1984-07       Impact factor: 3.411

7.  Detection and expression of DNA homologous to the tox gene in nontoxinogenic isolates of Corynebacterium diphtheriae.

Authors:  N Groman; N Cianciotto; M Bjorn; M Rabin
Journal:  Infect Immun       Date:  1983-10       Impact factor: 3.441
  7 in total
  44 in total

1.  Rapid enzyme immunoassay for determination of toxigenicity among clinical isolates of corynebacteria.

Authors:  K H Engler; A Efstratiou
Journal:  J Clin Microbiol       Date:  2000-04       Impact factor: 5.948

2.  Immunochromatographic strip test for rapid detection of diphtheria toxin: description and multicenter evaluation in areas of low and high prevalence of diphtheria.

Authors:  K H Engler; A Efstratiou; D Norn; R S Kozlov; I Selga; T G Glushkevich; M Tam; V G Melnikov; I K Mazurova; V E Kim; G Y Tseneva; L P Titov; R C George
Journal:  J Clin Microbiol       Date:  2002-01       Impact factor: 5.948

3.  Characterization of Corynebacterium species in macaques.

Authors:  Jaime Venezia; Pamela K Cassiday; Robert P Marini; Zeli Shen; Ellen M Buckley; Yaicha Peters; Nancy Taylor; Floyd E Dewhirst; Maria L Tondella; James G Fox
Journal:  J Med Microbiol       Date:  2012-06-21       Impact factor: 2.472

4.  Analysis of toxigenic Corynebacterium ulcerans strains revealing potential for false-negative real-time PCR results.

Authors:  Pamela K Cassiday; Lucia C Pawloski; Tejpratap Tiwari; Gary N Sanden; Patricia P Wilkins
Journal:  J Clin Microbiol       Date:  2007-11-07       Impact factor: 5.948

5.  Cutaneous diphtheria in the urban poor population of Vancouver, British Columbia, Canada: a 10-year review.

Authors:  C F Lowe; K A Bernard; M G Romney
Journal:  J Clin Microbiol       Date:  2011-04-27       Impact factor: 5.948

6.  Canada's first case of a multidrug-resistant Corynebacterium diphtheriae strain, isolated from a skin abscess.

Authors:  Neil V Mina; Tamara Burdz; Deborah Wiebe; Jagtar S Rai; Tazim Rahim; Fern Shing; Linda Hoang; Kathryn Bernard
Journal:  J Clin Microbiol       Date:  2011-08-31       Impact factor: 5.948

7.  Reversed passive latex agglutination assay for detection of toxigenic Corynebacterium diphtheriae.

Authors:  C Toma; L Sisavath; M Iwanaga
Journal:  J Clin Microbiol       Date:  1997-12       Impact factor: 5.948

Review 8.  The genus corynebacterium and other medically relevant coryneform-like bacteria.

Authors:  Kathryn Bernard
Journal:  J Clin Microbiol       Date:  2012-07-25       Impact factor: 5.948

9.  Human clinical isolates of Corynebacterium diphtheriae and Corynebacterium ulcerans collected in Canada from 1999 to 2003 but not fitting reporting criteria for cases of diphtheria.

Authors:  Leanne M Dewinter; Kathryn A Bernard; Marc G Romney
Journal:  J Clin Microbiol       Date:  2005-07       Impact factor: 5.948

10.  Comparison of four molecular typing methods for characterization of Corynebacterium diphtheriae and determination of transcontinental spread of C. diphtheriae based on BstEII rRNA gene profiles.

Authors:  Aruni De Zoysa; Peter Hawkey; Andre Charlett; Androulla Efstratiou
Journal:  J Clin Microbiol       Date:  2008-09-10       Impact factor: 5.948
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