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Literature DB >> 8576378

Application of PCR for detection of toxigenic Corynebacterium diphtheriae strains isolated during the Russian diphtheria epidemic, 1990 through 1994.

V M Mikhailovich¹, V G Melnikov, I K Mazurova, I K Wachsmuth, J D Wenger, M Wharton, H Nakao, T Popovic.

Abstract

A total of 250 Corynebacterium diphtheriae isolates from clinical cases and carriers in Russia were assayed by PCR directed at the A subunit of the diphtheria toxin gene to distinguish toxigenic from nontoxigenic strains; 170 strains were positive as indicated by the presence of the 248-bp amplicon. The results of this PCR assay were in complete concordance with those of the standard immunoprecipitation assay (Elek), and the PCR assay is a useful tool for rapid identification in clinical laboratories.

Entities: Species

Mesh：

Substances：
Diphtheria Toxin

Year: 1995 PMID： 8576378 PMCID： PMC228639 DOI： 10.1128/jcm.33.11.3061-3063.1995

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

6 in total

1. Rapid screening for toxigenic Corynebacterium diphtheriae by the polymerase chain reaction.

Authors: M J Pallen
Journal: J Clin Pathol Date: 1991-12 Impact factor: 3.411

2. Polymerase chain reaction assay for diagnosis of potentially toxinogenic Corynebacterium diphtheriae strains: correlation with ADP-ribosylation activity assay.

Authors: D Hauser; M R Popoff; M Kiredjian; P Boquet; F Bimet
Journal: J Clin Microbiol Date: 1993-10 Impact factor: 5.948

3. Are we ready for diphtheria? A report from the Diphtheria Diagnostic Workshop, Atlanta, 11 and 12 July 1994.

Authors: T Popovic; M Wharton; J D Wenger; L McIntyre; I K Wachsmuth
Journal: J Infect Dis Date: 1995-04 Impact factor: 5.226

4. Polymerase chain reaction for screening clinical isolates of corynebacteria for the production of diphtheria toxin.

Authors: M J Pallen; A J Hay; L H Puckey; A Efstratiou
Journal: J Clin Pathol Date: 1994-04 Impact factor: 3.411

5. Detection and expression of DNA homologous to the tox gene in nontoxinogenic isolates of Corynebacterium diphtheriae.

Authors: N Groman; N Cianciotto; M Bjorn; M Rabin
Journal: Infect Immun Date: 1983-10 Impact factor: 3.441

6. Nucleotide sequence of the structural gene for diphtheria toxin carried by corynebacteriophage beta.

Authors: L Greenfield; M J Bjorn; G Horn; D Fong; G A Buck; R J Collier; D A Kaplan
Journal: Proc Natl Acad Sci U S A Date: 1983-11 Impact factor: 11.205

6 in total

16 in total

1. Persistence of a distinct Corynebacterium diphtheriae clonal group within two communities in the United States and Canada where diphtheria is endemic.

Authors: C K Marston; F Jamieson; F Cahoon; G Lesiak; A Golaz; M Reeves; T Popovic
Journal: J Clin Microbiol Date: 2001-04 Impact factor: 5.948

2. Rapid enzyme immunoassay for determination of toxigenicity among clinical isolates of corynebacteria.

Authors: K H Engler; A Efstratiou
Journal: J Clin Microbiol Date: 2000-04 Impact factor: 5.948

3. Retrospective diagnosis of diphtheria by detection of the Corynebacterium diphtheriae tox gene in a formaldehyde-fixed throat swab using PCR and sequencing analysis.

Authors: T Komiya; N Shibata; M Ito; M Takahashi; Y Arakawa
Journal: J Clin Microbiol Date: 2000-06 Impact factor: 5.948

Application of PCR for detection of toxigenic Corynebacterium diphtheriae strains isolated during the Russian diphtheria epidemic, 1990 through 1994.

1. Rapid screening for toxigenic Corynebacterium diphtheriae by the polymerase chain reaction.

2. Polymerase chain reaction assay for diagnosis of potentially toxinogenic Corynebacterium diphtheriae strains: correlation with ADP-ribosylation activity assay.

3. Are we ready for diphtheria? A report from the Diphtheria Diagnostic Workshop, Atlanta, 11 and 12 July 1994.

4. Polymerase chain reaction for screening clinical isolates of corynebacteria for the production of diphtheria toxin.

5. Detection and expression of DNA homologous to the tox gene in nontoxinogenic isolates of Corynebacterium diphtheriae.

6. Nucleotide sequence of the structural gene for diphtheria toxin carried by corynebacteriophage beta.

1. Persistence of a distinct Corynebacterium diphtheriae clonal group within two communities in the United States and Canada where diphtheria is endemic.

2. Rapid enzyme immunoassay for determination of toxigenicity among clinical isolates of corynebacteria.

3. Retrospective diagnosis of diphtheria by detection of the Corynebacterium diphtheriae tox gene in a formaldehyde-fixed throat swab using PCR and sequencing analysis.

4. Persistence of nontoxigenic Corynebacterium diphtheriae biotype gravis strains in Pondicherry, Southern India.

Review 5. Respiratory diphtheria in the United States, 1980 through 1995.

6. Reversed passive latex agglutination assay for detection of toxigenic Corynebacterium diphtheriae.

7. Fatal respiratory tract diphtheria apparently caused by nontoxigenic strains of Corynebacterium diphtheriae.

8. A modified Elek test for detection of toxigenic corynebacteria in the diagnostic laboratory.

9. Development of a direct PCR assay for detection of the diphtheria toxin gene.

10. Molecular characterization of Corynebacterium diphtheriae isolates, Russia, 1957-1987.