Literature DB >> 8998994

PCR amplification of the rDNA internal transcribed spacer region for differentiation of Saccharomyces cultures.

P Valente1, F C Gouveia, G A de Lemos, D Pimentel, J D van Elsas, L C Mendonça-Hagler, A N Hagler.   

Abstract

The size of the internal transcribed spacer (ITS) region as measured by gel electrophoresis of PCR products, amplified by primers ITS1 and ITS4, was over 800 bp for all Saccharomyces sensu stricto species, but yeasts belonging to other Saccharomyces species had a shorter ITS region, making this characteristic potentially useful in the identification of Saccharomyces isolates. The ITS product length was homogeneous within the species Saccharomyces cerevisiae.

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Year:  1996        PMID: 8998994     DOI: 10.1111/j.1574-6968.1996.tb08114.x

Source DB:  PubMed          Journal:  FEMS Microbiol Lett        ISSN: 0378-1097            Impact factor:   2.742


  5 in total

1.  Primers ITS1, ITS2 and ITS4 detect the intraspecies variability in the internal transcribed spacers and 5.8S rRNA gene region in clinical isolates of fungi.

Authors:  M Korabecná; V Liska; K Fajfrlík
Journal:  Folia Microbiol (Praha)       Date:  2003       Impact factor: 2.099

2.  Biochemical and molecular characterization of Saccharomyces cerevisiae strains obtained from sugar-cane juice fermentations and their impact in cachaça production.

Authors:  Valdinéia Aparecida Oliveira; Maristela Araújo Vicente; Luciano Gomes Fietto; Ieso de Miranda Castro; Maurício Xavier Coutrim; Dorit Schüller; Henrique Alves; Margarida Casal; Juliana de Oliveira Santos; Leandro Dias Araújo; Paulo Henrique Alves da Silva; Rogelio Lopes Brandão
Journal:  Appl Environ Microbiol       Date:  2007-12-07       Impact factor: 4.792

3.  Molecular characterization of Yarrowia lipolytica and Candida zeylanoides isolated from poultry.

Authors:  T Deak; J Chen; L R Beuchat
Journal:  Appl Environ Microbiol       Date:  2000-10       Impact factor: 4.792

4.  Kluyveromyces aestuarii, a potential environmental quality indicator yeast for mangroves in the State of Rio de Janeiro, Brazil.

Authors:  F V Araujo; A N Hagler
Journal:  Braz J Microbiol       Date:  2011-09-01       Impact factor: 2.476

5.  Petroleum contamination and bioaugmentation in bacterial rhizosphere communities from Avicennia schaueriana.

Authors:  Simone Dealtry; Angela Michelato Ghizelini; Leda C S Mendonça-Hagler; Ricardo Moreira Chaloub; Fernanda Reinert; Tácio M P de Campos; Newton C M Gomes; Kornelia Smalla
Journal:  Braz J Microbiol       Date:  2018-06-14       Impact factor: 2.476

  5 in total

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