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Literature DB >> 8991639

Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein.

M Schmidt¹, C Lindqvist, A Salmi, C Oker-Blom.

Abstract

The structural proteins of rubella virus (RV) were expressed in insect cells by using the baculovirus expression vector system. The recombinant E1 envelope glycoprotein was purified by immunoaffinity chromatography and used to detect RV-specific immunoglobulin M antibodies in a time-resolved fluoroimmunoassay. Correlation analysis between the reactivities of antibodies against this recombinant E1 and the reactivities against authentic RV antigen shows that purified E1 can detect RV antibodies of the immunoglobulin M type.

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Year: 1996 PMID： 8991639 PMCID： PMC170281 DOI： 10.1128/cdli.3.2.216-218.1996

Source DB: PubMed Journal: Clin Diagn Lab Immunol ISSN： 1071-412X

36 in total

1. New sensitive and specific assay for human immunodeficiency virus antibodies using labeled recombinant fusion protein and time-resolved fluoroimmunoassay.

Authors: H Siitari; P Turunen; J Schrimsher; M Nunn
Journal: J Clin Microbiol Date: 1990-09 Impact factor: 5.948

2. Structure of rubella E1 glycoprotein epitopes established by multiple peptide synthesis.

Authors: L Lozzi; M Rustici; M Corti; M G Cusi; P E Valensin; L Bracci; A Santucci; P Soldani; A Spreafico; P Neri
Journal: Arch Virol Date: 1990 Impact factor: 2.574

3. Rubella virus antigens: localization of epitopes involved in hemagglutination and neutralization by using monoclonal antibodies.

Authors: K Y Green; P H Dorsett
Journal: J Virol Date: 1986-03 Impact factor: 5.103

4. Baculovirus polyhedrin promoter-directed expression of rubella virus envelope glycoproteins, E1 and E2, in Spodoptera frugiperda cells.

Authors: C Oker-Blom; R F Pettersson; M D Summers
Journal: Virology Date: 1989-09 Impact factor: 3.616

5. Vaccinia-vectored expression of the rubella virus structural proteins and characterization of the E1 and E2 glycosidic linkages.

Authors: A Sanchez; T K Frey
Journal: Virology Date: 1991-08 Impact factor: 3.616

6. Comparison of a simple latex agglutination test with hemolysis-in-gel, hemagglutination inhibition, and radioimmunoassay for detection of rubella virus antibodies.

Authors: P Väänänen; V M Häivä; P Koskela; O Meurman
Journal: J Clin Microbiol Date: 1985-05 Impact factor: 5.948

Detection of rubella virus-specific immunoglobulin M antibodies with a baculovirus-expressed E1 protein.

1. New sensitive and specific assay for human immunodeficiency virus antibodies using labeled recombinant fusion protein and time-resolved fluoroimmunoassay.

2. Structure of rubella E1 glycoprotein epitopes established by multiple peptide synthesis.

3. Rubella virus antigens: localization of epitopes involved in hemagglutination and neutralization by using monoclonal antibodies.

4. Baculovirus polyhedrin promoter-directed expression of rubella virus envelope glycoproteins, E1 and E2, in Spodoptera frugiperda cells.

5. Vaccinia-vectored expression of the rubella virus structural proteins and characterization of the E1 and E2 glycosidic linkages.

6. Comparison of a simple latex agglutination test with hemolysis-in-gel, hemagglutination inhibition, and radioimmunoassay for detection of rubella virus antibodies.

Review 7. Insect baculoviruses: powerful gene expression vectors.

8. A bio-engineered rubella E1 antigen.

9. Processing and intracellular transport of rubella virus structural proteins in COS cells.

10. Sequence of the genome RNA of rubella virus: evidence for genetic rearrangement during togavirus evolution.

1. Preliminary multiplex microarray IgG immunoassay for the diagnosis of toxoplasmosis and rubella.