Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Nucleotide sequences of nuclear tRNA(Cys) genes from Nicotiana and Arabidopsis and expression in HeLa cell extract.

Literature DB >> 8980505

Nucleotide sequences of nuclear tRNA(Cys) genes from Nicotiana and Arabidopsis and expression in HeLa cell extract.

Abstract

We have recently characterized Nicotiana cytoplasmic (cyt) tRNA(GCA)Cys as a novel UGA suppressor tRNA. Here we have isolated its corresponding (NtC1) and a variant (NtC2) gene from a genomic library of Nicotiana rustica. Both tRNA(Cys) genes are efficiently transcribed in HeLa cell nuclear extract and yield mature cyt tRNAs(Cys). Sequence analysis of the upstream region of the RAD51 single-copy gene of the Arabidopsis thaliana genome revealed a cluster of three tRNA(Cys) genes which have the same polarity and comprise highly similar flanking sequences. Of the three Arabidopsis tRNA(Cys) genes only one (i.e. AtC2) appears to code for a functional gene which exhibits an almost identical nucleotide sequence to NtC1. These are the first sequenced nuclear tDNAs(Cys) of plant origin.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1996 PMID： 8980505 DOI： 10.1007/bf00019108

Source DB: PubMed Journal: Plant Mol Biol ISSN： 0167-4412 Impact factor: 4.076

14 in total

1. Expression of variant nuclear Arabidopsis tRNA(Ser) genes and pre-tRNA maturation differ in HeLa, yeast and wheat germ extracts.

Authors: D Beier; H Beier
Journal: Mol Gen Genet Date: 1992-05

2. Genes, variant genes and pseudogenes of the human tRNA(Val) gene family. Expression and pre-tRNA maturation in vitro.

Authors: H U Thomann; C Schmutzler; U Hüdepohl; M Blow; H J Gross
Journal: J Mol Biol Date: 1989-10-20 Impact factor: 5.469

Review 3. Transcription by RNA polymerase III.

Authors: E P Geiduschek; G P Tocchini-Valentini
Journal: Annu Rev Biochem Date: 1988 Impact factor: 23.643

4. The tRNA(Ser)-isoacceptors and their genes in Nicotiana rustica: genome organization, expression in vitro and sequence analyses.

Authors: T Teichmann; C Urban; H Beier
Journal: Plant Mol Biol Date: 1994-03 Impact factor: 4.076

5. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.

Authors: J D Dignam; R M Lebovitz; R G Roeder
Journal: Nucleic Acids Res Date: 1983-03-11 Impact factor: 16.971

6. Cysteine tRNAs of plant origin as novel UGA suppressors.

Authors: C Urban; H Beier
Journal: Nucleic Acids Res Date: 1995-11-25 Impact factor: 16.971

7. The presence of tRNA pseudogenes in mammalia and plants and their absence in yeast may account for different specificities of pre-tRNA processing enzymes.

Authors: Y Kaçar; H Beier; H J Gross
Journal: Gene Date: 1995-04-14 Impact factor: 3.688

8. The tRNATyr multigene family of Triticum aestivum: genome organization, sequence analyses and maturation of intron-containing pre-tRNAs in wheat germ extract.

Authors: S Arends; J Kraus; H Beier
Journal: FEBS Lett Date: 1996-04-22 Impact factor: 4.124

9. A cell-free plant extract for accurate pre-tRNA processing, splicing and modification.

Authors: N Stange; H Beier
Journal: EMBO J Date: 1987-09 Impact factor: 11.598

10. A human and a plant intron-containing tRNATyr gene are both transcribed in a HeLa cell extract but spliced along different pathways.

Authors: H van Tol; N Stange; H J Gross; H Beier
Journal: EMBO J Date: 1987-01 Impact factor: 11.598

1 in total

1. Nucleotide sequences and functional characterization of two tobacco UAG suppressor tRNA(Gln) isoacceptors and their genes.

Authors: M Grimm; A Nass; C Schüll; H Beier
Journal: Plant Mol Biol Date: 1998-11 Impact factor: 4.076

1 in total