Literature DB >> 8979358

Purification and characterization of a prolidase from Lactobacillus casei subsp. casei IFPL 731.

M D Fernández-Esplá1, M C Martín-Hernández, P F Fox.   

Abstract

A peptidase showing a high level of specificity towards dipeptides of the X-Pro type was purified to homogeneity from the cell extract of Lactobacillus casei subsp. casei IFPL 731. The enzyme was a monomer having a molecular mass of 41 kDa. The pH and temperature optima were 6.5 to 7.5 and 55 degrees C, respectively. Metal chelating agents completely inhibited enzyme activity, indicating that the prolidase was a metalloenzyme. The Michaelis constant (K(m)) and Vmax for several proline-containing dipeptides were determined.

Entities:  

Mesh：

Substances：

Year:  1997        PMID： 8979358      PMCID： PMC168322          DOI： 10.1128/aem.63.1.314-316.1997

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  Purification and characterization of an aminoacyl proline hydrolase from guinea-pig intestinal mucosa.

Authors:  G O'Cuinn; P F Fottrell
Journal:  Biochim Biophys Acta       Date:  1975-06-24

2.  Purification and Characterization of a Dipeptidase from Streptococcus cremoris Wg2.

Authors:  A van Boven; P S T Tan; W N Konings
Journal:  Appl Environ Microbiol       Date:  1988-01       Impact factor: 4.792

3.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

4.  Purification and specificity of pig intestinal prolidase.

Authors:  H Sjöström; O Norén; L Josefsson
Journal:  Biochim Biophys Acta       Date:  1973-12-19

5.  Cloning and DNA sequence analysis of pepQ, a prolidase gene from Lactobacillus delbrueckii subsp. lactis DSM7290 and partial characterization of its product.

Authors:  K Stucky; J R Klein; A Schüller; H Matern; B Henrich; R Plapp
Journal:  Mol Gen Genet       Date:  1995-05-20

6.  Modified colorimetric ninhydrin methods for peptidase assay.

Authors:  E Doi; D Shibata; T Matoba
Journal:  Anal Biochem       Date:  1981-11-15       Impact factor: 3.365

7.  Purification of X-prolyl dipeptidyl aminopeptidase from Lactobacillus casei subspecies.

Authors:  M el Abboudi; M el Soda; S Pandian; R E Simard; N F Olson
Journal:  Int J Food Microbiol       Date:  1992 Jan-Feb       Impact factor: 5.277

8.  Prolidase from bovine intestine: purification and characterization.

Authors:  T Yoshimoto; F Matsubara; E Kawano; D Tsuru
Journal:  J Biochem       Date:  1983-12       Impact factor: 3.387

9.  Purification and characterization of X-prolyl dipeptidyl peptidase from Lactobacillus casei subsp. casei LLG.

Authors:  M B Habibi-Najafi; B H Lee
Journal:  Appl Microbiol Biotechnol       Date:  1994-11       Impact factor: 4.813

10.  [Peptide hydrolases of lactobacilli of the Thermobacterium group. I. Demonstration of these activities in Lactobacillus helveticus, L. acidophilus, L. lactis and L. bulgaricus].

Authors:  M El Soda; M J Desmazeaud
Journal:  Can J Microbiol       Date:  1982-10       Impact factor: 2.419
  10 in total
  12 in total

Review 1.  Lactic acid bacteria as a potential source of enzymes for use in vinification.

Authors:  Angela Matthews; Antonio Grimaldi; Michelle Walker; Eveline Bartowsky; Paul Grbin; Vladimir Jiranek
Journal:  Appl Environ Microbiol       Date:  2004-10       Impact factor: 4.792

2.  Identifying the structure of the active sites of human recombinant prolidase.

Authors:  Roberta Besio; Stefania Alleva; Antonella Forlino; Anna Lupi; Carlo Meneghini; Velia Minicozzi; Antonella Profumo; Francesco Stellato; Ruggero Tenni; Silvia Morante
Journal:  Eur Biophys J       Date:  2009-05-05       Impact factor: 1.733

3.  X-prolyl dipeptidyl aminopeptidase gene (pepX) is part of the glnRA operon in Lactobacillus rhamnosus.

Authors:  P Varmanen; K Savijoki; S Avall; A Palva; S Tynkkynen
Journal:  J Bacteriol       Date:  2000-01       Impact factor: 3.490

4.  Cloning and characterization of a prolinase gene (pepR) from Lactobacillus rhamnosus.

Authors:  P Varmanen; T Rantanen; A Palva; S Tynkkynen
Journal:  Appl Environ Microbiol       Date:  1998-05       Impact factor: 4.792

5.  Molecular characterisation of six patients with prolidase deficiency: identification of the first small duplication in the prolidase gene and of a mutation generating symptomatic and asymptomatic outcomes within the same family.

Authors:  A Lupi; A Rossi; E Campari; F Pecora; A M Lund; N H Elcioglu; M Gultepe; M Di Rocco; G Cetta; A Forlino
Journal:  J Med Genet       Date:  2006-12       Impact factor: 6.318

6.  Characterization of native and recombinant forms of an unusual cobalt-dependent proline dipeptidase (prolidase) from the hyperthermophilic archaeon Pyrococcus furiosus.

Authors:  M Ghosh; A M Grunden; D M Dunn; R Weiss; M W Adams
Journal:  J Bacteriol       Date:  1998-09       Impact factor: 3.490

7.  Crystal Structural and Functional Analysis of the Putative Dipeptidase from Pyrococcus horikoshii OT3.

Authors:  Jeyaraman Jeyakanthan; Katsumi Takada; Masahide Sawano; Kyoko Ogasahara; Hisashi Mizutani; Naoki Kunishima; Shigeyuki Yokoyama; Katsuhide Yutani
Journal:  J Biophys       Date:  2009-06-28

8.  Hydrolysis of sequenced beta-casein peptides provides new insight into peptidase activity from thermophilic lactic acid bacteria and highlights intrinsic resistance of phosphopeptides.

Authors:  S M Deutsch; D Molle; V Gagnaire; M Piot; D Atlan; S Lortal
Journal:  Appl Environ Microbiol       Date:  2000-12       Impact factor: 4.792

9.  Proline iminopeptidase PepI overexpressing Lactobacillus casei as an adjunct starter in Edam cheese.

Authors:  Sahar Navidghasemizad; Timo M Takala; Tapani Alatossava; Per Ej Saris
Journal:  Bioengineered       Date:  2013-07-12       Impact factor: 3.269

10.  Structural basis of substrate selectivity of E. coli prolidase.

Authors:  Jeremy Weaver; Tylan Watts; Pingwei Li; Hays S Rye
Journal:  PLoS One       Date:  2014-10-29       Impact factor: 3.240
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.