BACKGROUND: The growth and function the normal prostate is dependent on the presence of androgen. As prostate tumors progress there is a loss of androgen-dependent cell growth. The identification of the genes that are regulated by androgens may be of pathological and clinical significance. METHODS: In this study the differential display method was used to identify genes regulated by androgen in an androgen-responsive prostate cancer cell line, LNCaP-FGC. RESULTS: A gene whose expression is down-regulated in LNCaP-FGC cells after 30 hr of androgen deprivation has been identified. This gene is a previously identified member of the S100 gene family of calcium-binding proteins, namely S100P. Here we show that S100P expression is regulated by the synthetic androgen R1881, but not by serum growth factors. It is dysregulated in the androgen-independent prostate cancer cell lines LNCaP-R, DU145, and PC3. CONCLUSIONS: The data indicate that S100P may play a role in the etiology of prostate cancer.
BACKGROUND: The growth and function the normal prostate is dependent on the presence of androgen. As prostate tumors progress there is a loss of androgen-dependent cell growth. The identification of the genes that are regulated by androgens may be of pathological and clinical significance. METHODS: In this study the differential display method was used to identify genes regulated by androgen in an androgen-responsive prostate cancer cell line, LNCaP-FGC. RESULTS: A gene whose expression is down-regulated in LNCaP-FGC cells after 30 hr of androgen deprivation has been identified. This gene is a previously identified member of the S100 gene family of calcium-binding proteins, namely S100P. Here we show that S100P expression is regulated by the synthetic androgen R1881, but not by serum growth factors. It is dysregulated in the androgen-independent prostate cancer cell lines LNCaP-R, DU145, and PC3. CONCLUSIONS: The data indicate that S100P may play a role in the etiology of prostate cancer.
Authors: Yi-Chien Lee; David E Volk; Varatharasa Thiviyanathan; Quinn Kleerekoper; Alexey V Gribenko; Shanmin Zhang; David G Gorenstein; George I Makhatadze; Bruce A Luxon Journal: J Biomol NMR Date: 2004-07 Impact factor: 2.835
Authors: Guozheng Wang; Shu Zhang; David G Fernig; David Spiller; Marisa Martin-Fernandez; Hongmei Zhang; Yi Ding; Zihe Rao; Philip S Rudland; Roger Barraclough Journal: Biochem J Date: 2004-08-15 Impact factor: 3.857
Authors: Xiaofei Chang; Constance L Monitto; Semra Demokan; Myoung Sook Kim; Steven S Chang; Xiaoli Zhong; Joseph A Califano; David Sidransky Journal: Cancer Res Date: 2010-03-09 Impact factor: 12.701