Literature DB >> 8972875

A tri-hybrid system for the analysis and detection of RNA--protein interactions.

U Putz1, P Skehel, D Kuhl.   

Abstract

A modification of the two-hybrid system is described for the in vivo reconstruction of specific RNA-protein interactions. In this tri-hybrid system, the DNA binding and transcription activation domains of the yeast transcriptional activator GAL4 are brought together via the interaction of recombinant fusion proteins with a recombinant RNA. The method provides a system for studying RNA-protein interactions with the genetic advantages of the two-hybrid system. It may be used to detect specific RNA-binding proteins or target RNAs from a library of cDNAs, or to analyse the structural specificity of identified RNA-protein interactions.

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Year:  1996        PMID： 8972875      PMCID： PMC146291          DOI： 10.1093/nar/24.23.4838

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  18 in total

1.  A three-hybrid system to detect RNA-protein interactions in vivo.

Authors:  D J SenGupta; B Zhang; B Kraemer; P Pochart; S Fields; M Wickens
Journal:  Proc Natl Acad Sci U S A       Date:  1996-08-06       Impact factor: 11.205

2.  Sequence-specific RNA binding by the HIV-1 Rev protein.

Authors:  M L Zapp; M R Green
Journal:  Nature       Date:  1989-12-07       Impact factor: 49.962

3.  Functional dissection of the HIV-1 Rev trans-activator--derivation of a trans-dominant repressor of Rev function.

Authors:  M H Malim; S Böhnlein; J Hauber; B R Cullen
Journal:  Cell       Date:  1989-07-14       Impact factor: 41.582

4.  Effects of expression of mammalian G alpha and hybrid mammalian-yeast G alpha proteins on the yeast pheromone response signal transduction pathway.

Authors:  Y S Kang; J Kane; J Kurjan; J M Stadel; D J Tipper
Journal:  Mol Cell Biol       Date:  1990-06       Impact factor: 4.272

5.  HIV-1 structural gene expression requires binding of the Rev trans-activator to its RNA target sequence.

Authors:  M H Malim; L S Tiley; D F McCarn; J R Rusche; J Hauber; B R Cullen
Journal:  Cell       Date:  1990-02-23       Impact factor: 41.582

6.  Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate-dehydrogenase multigenic family.

Authors:  P Fort; L Marty; M Piechaczyk; S el Sabrouty; C Dani; P Jeanteur; J M Blanchard
Journal:  Nucleic Acids Res       Date:  1985-03-11       Impact factor: 16.971

7.  A novel genetic system to detect protein-protein interactions.

Authors:  S Fields; O Song
Journal:  Nature       Date:  1989-07-20       Impact factor: 49.962

8.  Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations.

Authors:  S J Elledge; J T Mulligan; S W Ramer; M Spottswood; R W Davis
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-01       Impact factor: 11.205

9.  The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases.

Authors:  J W Harper; G R Adami; N Wei; K Keyomarsi; S J Elledge
Journal:  Cell       Date:  1993-11-19       Impact factor: 41.582

10.  Specific binding of HIV-1 recombinant Rev protein to the Rev-responsive element in vitro.

Authors:  T J Daly; K S Cook; G S Gray; T E Maione; J R Rusche
Journal:  Nature       Date:  1989-12-14       Impact factor: 49.962
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  20 in total

1.  Positive and negative mutant selection in the human histone hairpin-binding protein using the yeast three-hybrid system.

Authors:  F Martin; F Michel; D Zenklusen; B Müller; D Schümperli
Journal:  Nucleic Acids Res       Date:  2000-04-01       Impact factor: 16.971

2.  The bI4 group I intron binds directly to both its protein splicing partners, a tRNA synthetase and maturase, to facilitate RNA splicing activity.

Authors:  S B Rho; S A Martinis
Journal:  RNA       Date:  2000-12       Impact factor: 4.942

3.  Interactions of Escherichia coli RNA with bacteriophage MS2 coat protein: genomic SELEX.

Authors:  T Shtatland; S C Gill; B E Javornik; H E Johansson; B S Singer; O C Uhlenbeck; D A Zichi; L Gold
Journal:  Nucleic Acids Res       Date:  2000-11-01       Impact factor: 16.971

4.  T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from cDNA libraries.

Authors:  S Danner; J G Belasco
Journal:  Proc Natl Acad Sci U S A       Date:  2001-10-23       Impact factor: 11.205

5.  Two distinct domains of the beta subunit of Aquifex aeolicus leucyl-tRNA synthetase are involved in tRNA binding as revealed by a three-hybrid selection.

Authors:  Yong-Gang Zheng; Hui Wei; Chen Ling; Franck Martin; Gilbert Eriani; En-Duo Wang
Journal:  Nucleic Acids Res       Date:  2004-06-18       Impact factor: 16.971

6.  The Apc5 subunit of the anaphase-promoting complex/cyclosome interacts with poly(A) binding protein and represses internal ribosome entry site-mediated translation.

Authors:  Nadejda Koloteva-Levine; Dalia Pinchasi; Idan Pereman; Amit Zur; Michael Brandeis; Orna Elroy-Stein
Journal:  Mol Cell Biol       Date:  2004-05       Impact factor: 4.272

Review 7.  Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.

Authors:  Bram Stynen; Hélène Tournu; Jan Tavernier; Patrick Van Dijck
Journal:  Microbiol Mol Biol Rev       Date:  2012-06       Impact factor: 11.056

8.  Evolution of a split RNA polymerase as a versatile biosensor platform.

Authors:  Jinyue Pu; Julia Zinkus-Boltz; Bryan C Dickinson
Journal:  Nat Chem Biol       Date:  2017-02-13       Impact factor: 15.040

9.  A translational repression assay procedure (TRAP) for RNA-protein interactions in vivo.

Authors:  E Paraskeva; A Atzberger; M W Hentze
Journal:  Proc Natl Acad Sci U S A       Date:  1998-02-03       Impact factor: 11.205

10.  Detection and isolation of RNA-binding proteins by RNA-ligand screening of a cDNA expression library.

Authors:  R Sägesser; E Martinez; M Tsagris; M Tabler
Journal:  Nucleic Acids Res       Date:  1997-10-01       Impact factor: 16.971
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