Literature DB >> 896485

Partial purification of rat alpha-lactalbumin mRNA.

P K Chakrabartty, P K Qasba.   

Abstract

Alpha-lactalbumin messenger RNA was partially purified from RNA extracted from 3-5 day lactating rat mammary glands on a poly(U)-sepharose column followed by sucrose gradient centrifugation. Apha-Lactalbumin mRNA activity was assayed in wheat germ cell-free translational system by immunoprecipitation of the in vitro synthesized protein using specific antiserum prepared against purified rat alpha-lactalbumin. In the purified mRNA preparation alpha-lactalbumin mRNA activity comprised approximately 85% of the total mRNA activity.

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Year:  1977        PMID： 896485      PMCID： PMC342543          DOI： 10.1093/nar/4.6.2065

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  Fractionation of nucleic acids with special regard to rapidly labeled RNA.

Authors:  H Kern
Journal:  Anal Biochem       Date:  1975-07       Impact factor: 3.365

2.  Regulation of casein messenger RNA during the development of the rat mammary gland.

Authors:  J M Rosen; S L Woo; J P Comstock
Journal:  Biochemistry       Date:  1975-07       Impact factor: 3.162

3.  The isolation of mRNA encoding the alpha subunit of human chorionic gonadotropin.

Authors:  T D Landefeld; D R McWilliams; I Boime
Journal:  Biochem Biophys Res Commun       Date:  1976-09-20       Impact factor: 3.575

4.  Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.

Authors:  B E Roberts; B M Paterson
Journal:  Proc Natl Acad Sci U S A       Date:  1973-08       Impact factor: 11.205

5.  Isolation of rabbit reticulocyte 9 S mRNA.

Authors:  B Lebleu
Journal:  Methods Enzymol       Date:  1974       Impact factor: 1.600

6.  Analytical studies on nuclear ribonucleic acid using polyacrylamide gel electrophoresis.

Authors:  C W Dingman; A C Peacock
Journal:  Biochemistry       Date:  1968-02       Impact factor: 3.162

7.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

8.  Resolution of a soluble lactose synthetase into two protein components and solubilization of microsomal lactose synthetase.

Authors:  U Brodbeck; K E Ebner
Journal:  J Biol Chem       Date:  1966-02-10       Impact factor: 5.157

9.  Cell-free synthesis of rat preproinsulins: characterization and partial amino acid sequence determination.

Authors:  S J Chan; P Keim; D F Steiner
Journal:  Proc Natl Acad Sci U S A       Date:  1976-06       Impact factor: 11.205

10.  Isolation and characterization of adenovirus messenger ribonucleic acid in productive infection.

Authors:  U Lindberg; T Persson; L Philipson
Journal:  J Virol       Date:  1972-11       Impact factor: 5.103
  10 in total
  4 in total

1.  Complete sequence analysis of cDNA clones encoding rat whey phosphoprotein: homology to a protease inhibitor.

Authors:  A M Dandekar; E A Robinson; E Appella; P K Qasba
Journal:  Proc Natl Acad Sci U S A       Date:  1982-07       Impact factor: 11.205

2.  alpha-Lactalbumin mRNA in 4-day lactating rat mammary gland.

Authors:  P K Qasba; H L Nakhasi
Journal:  Proc Natl Acad Sci U S A       Date:  1978-10       Impact factor: 11.205

3.  Rat alpha-lactalbumin has a 17-residue-long COOH-terminal hydrophobic extension as judged by sequence analysis of the cDNA clones.

Authors:  A M Dandekar; P K Qasba
Journal:  Proc Natl Acad Sci U S A       Date:  1981-08       Impact factor: 11.205

4.  Comparative sequence analysis of the mRNAs coding for mouse and rat whey protein.

Authors:  L G Hennighausen; A E Sippel; A A Hobbs; J M Rosen
Journal:  Nucleic Acids Res       Date:  1982-06-25       Impact factor: 16.971
  4 in total

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